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Cloning and molecular characterization of a delta-6 fatty acid desaturase gene from Isochrysis sp CCMM5001
Wang, Shuai1,2; Zheng, Li2; Cui, Zhisong2; Chen, Junhui2; Yang, Baijuan2; Han, Xiaotian3; Liu, Chenguang1
2016-04-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
卷号28期号:2页码:921-929
文章类型Article
摘要The cDNA of the delta-6 (Delta 6) fatty acid desaturase gene IsFAD6 was isolated from the marine microalga Isochrysis sp. CCMM5001. Sequence analysis indicated that the total length of IsFAD6 is 2767 bp, and the gene contains a 1407-bp open reading frame, a 406-bp 5' untranslated region (UTR), and a 954-bp 3' UTR with a typical polyA tail. The gene encodes a protein with 469 amino acids, with a molecular weight of 52.9 kDa and an isoelectric point of 8.6. IsFAD6 contains three conserved histidine motifs and a cytochrome b5 domain. Real-time PCR analysis showed that the expression levels of IsFAD6 were significantly upregulated under diverse abiotic stresses, including low temperature (15 and 25 A degrees C), high salinity (62aEuro degrees), and high nitrogen deficiency (220 mu mol L-1 sodium nitrate), compared with those under control conditions (20 A degrees C, 31aEuro degrees salinity, and 880 mu mol L-1 sodium nitrate in the f/2 medium). The IsFAD6 transcript levels minimally increased under high salinity (93aEuro degrees) and excess sodium nitrate (1760 mu mol L-1). Our findings provided an understanding of IsFAD6 responses to temperature, salinity, and nitrate stresses at the transcriptional level. These results indicated that IsFAD6 cloned from Isochrysis sp. CCMM5001 is involved in acclimation to temperature, salt, and nitrogen stresses. The heterologous expression of IsFAD6 in Saccharomyces cerevisiae futher indicated that IsFAD6 could utilize endogenous substrates to synthesize gamma-linolenic acid (18:3(Delta 6,9,12)) and stearidonic acid (18:4(Delta 6,9,12,15)), which are involved in acclimation to adverse conditions.
关键词Isochrysis Sp. Delta-6 Fatty Acid Desaturase Gene Expression Analysis
DOI10.1007/s10811-015-0623-4
收录类别SCI
语种英语
WOS记录号WOS:000372256900021
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/126215
专题海洋生态与环境科学重点实验室
作者单位1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
2.State Ocean Adm, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
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Wang, Shuai,Zheng, Li,Cui, Zhisong,et al. Cloning and molecular characterization of a delta-6 fatty acid desaturase gene from Isochrysis sp CCMM5001[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(2):921-929.
APA Wang, Shuai.,Zheng, Li.,Cui, Zhisong.,Chen, Junhui.,Yang, Baijuan.,...&Liu, Chenguang.(2016).Cloning and molecular characterization of a delta-6 fatty acid desaturase gene from Isochrysis sp CCMM5001.JOURNAL OF APPLIED PHYCOLOGY,28(2),921-929.
MLA Wang, Shuai,et al."Cloning and molecular characterization of a delta-6 fatty acid desaturase gene from Isochrysis sp CCMM5001".JOURNAL OF APPLIED PHYCOLOGY 28.2(2016):921-929.
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