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CDNA CLONING, CHARACTERIZATION AND MRNA EXPRESSION OF CRYPTOCYANIN FROM THE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS H. MILNE EDWARDS, 1853
Wang, Xingqiang1,2; Wang, Lingling3; Wang, Mengqiang3; Zhang, Huan3; Song, Linsheng1,3
2016
发表期刊CRUSTACEANA
卷号89期号:3页码:273-290
文章类型Article
摘要Cryptocyanin is a copper-free non-respiratory protein and plays an important role in the physiological process of decapod crustaceans. In the present study, the full length cDNA encoding cryptocyanin (EsCc) was cloned from the haemocytes of the Chinese mitten crab Eriocheir sinensis, which was 2630 bp, consisting of a 5'-untranslated region of 237 bp, a 3'-untranslated region of 359 bp, and an open reading frame of 2034 bp. The deduced protein possessed 678 amino-acid residues, and the predicted molecular mass was 79.09 kDa. The phylogenetic analysis revealed that it belonged to the arthropod haemocyanin superfamily and shared 78% homology to Dungeness crab Metacarcinus magister cryptocyanin subunit 2. The expression pattern of EsCc in five tissues was investigated in premoult, intermoult and postmoult stages to understand its role during the moulting cycle. In hepatopancreas, the significant up-regulation of EsCc expression was observed in both premoult and intermoult stages, while the significant change of EsCc expressions in muscle and haemocytes was only found in premoult stages. To gain insight into the antimicrobial activity of EsCc in inflammatory processes, its expression profiles in the haemolymph of Chinese mitten crab challenged with Vibrio anguillarum and Pichia pastoris were examined. The challenge of V. anguillarum induced higher expression of EsCc as early as 6 h post-infection. Instead, a significant up-regulation of EsCc transcript was observed at 12 h post P. pastoris challenge. The above results implied that crustacean cryptocyanin was involved in the new exoskeleton formation. Also, it might be induced to resist invasions of pathogens.
关键词Cryptocyanin Eriocheir Sinensis Mrna Expression Profile Bacteria Challenge Immune Response
DOI10.1163/15685403-00003522
收录类别SCI
语种英语
WOS记录号WOS:000372355400002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/126207
专题实验海洋生物学重点实验室
作者单位1.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, 52 Heishijiao St, Dalian 116023, Peoples R China
2.Huaihai Inst Technol, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
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Wang, Xingqiang,Wang, Lingling,Wang, Mengqiang,et al. CDNA CLONING, CHARACTERIZATION AND MRNA EXPRESSION OF CRYPTOCYANIN FROM THE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS H. MILNE EDWARDS, 1853[J]. CRUSTACEANA,2016,89(3):273-290.
APA Wang, Xingqiang,Wang, Lingling,Wang, Mengqiang,Zhang, Huan,&Song, Linsheng.(2016).CDNA CLONING, CHARACTERIZATION AND MRNA EXPRESSION OF CRYPTOCYANIN FROM THE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS H. MILNE EDWARDS, 1853.CRUSTACEANA,89(3),273-290.
MLA Wang, Xingqiang,et al."CDNA CLONING, CHARACTERIZATION AND MRNA EXPRESSION OF CRYPTOCYANIN FROM THE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS H. MILNE EDWARDS, 1853".CRUSTACEANA 89.3(2016):273-290.
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