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Variation analysis of pathogenic Vibrio spp. and Pseudomonas spp. in Changhai mollusc farming waters using real-time PCR assay during 2011-2014
Liu, Rui1; Qiu, Limei1; Zhao, Xin1; Zhang, Huan1; Wang, Lingling1; Hou, Zhanhui1; Gao, Dahai1; Song, Linsheng2
2016
Source PublicationMARINE BIOLOGY RESEARCH
Volume12Issue:2Pages:146-157
SubtypeArticle
AbstractBacterial disease has caused high mortality of breeding molluscs from 2009 to 2011 in the Changhai area (Dalian, China). Vibrio spp. and Pseudomonas spp. have been detected as major pathogenic agents for aquatic animals in this area. In the present study, four virulence genes including vsm, toxR, aprX and carA were targeted to develop a real-time PCR assay for the quantitative detection of Vibrio splendidus, V. parahaemolyticus, Pseudomonas fluorescens and P. putida, respectively. The sensitivity and specificity of the assays were verified by experimental samples, and the variation tendencies of pathogenic V. splendidus, V. parahaemolyticus, P. fluorescens and P. putida strains from June to September were also detected in mollusc farming waters during 2011-2014. The concentration of V. splendidus increased from June to July, reduced in August, and then increased again in September. The highest count of V. parahaemolyticus appeared in July, and then dramatically decreased from August to September. Conversely, the counts of P. fluorescens and P. putida remained at lower levels from June to August, and then dramatically peaked in September. All four pathogenic bacteria displayed similar fluctuation tendencies of count variation in each year, and their concentrations were found to have a correlation with the average annual temperature. The variation tendency of pathogenic bacteria with temperature suggested that temperature was one of the most important factors to regulate the bacterial growth in a farming area, which could further provide information for the early warning of disease outbreak by using a convenient real-time PCR assay.
KeywordHongyue Dang Vibrio Splendidus Vibrio Parahaemolyticus Pseudomonas Fluorescens Pseudomonas Putida Pathogenic Bacteria Variation Tendency Real-time Pcr
DOI10.1080/17451000.2015.1099679
Indexed BySCI
Language英语
WOS IDWOS:000372363500004
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/126198
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Rui,Qiu, Limei,Zhao, Xin,et al. Variation analysis of pathogenic Vibrio spp. and Pseudomonas spp. in Changhai mollusc farming waters using real-time PCR assay during 2011-2014[J]. MARINE BIOLOGY RESEARCH,2016,12(2):146-157.
APA Liu, Rui.,Qiu, Limei.,Zhao, Xin.,Zhang, Huan.,Wang, Lingling.,...&Song, Linsheng.(2016).Variation analysis of pathogenic Vibrio spp. and Pseudomonas spp. in Changhai mollusc farming waters using real-time PCR assay during 2011-2014.MARINE BIOLOGY RESEARCH,12(2),146-157.
MLA Liu, Rui,et al."Variation analysis of pathogenic Vibrio spp. and Pseudomonas spp. in Changhai mollusc farming waters using real-time PCR assay during 2011-2014".MARINE BIOLOGY RESEARCH 12.2(2016):146-157.
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