| Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina | |
| Shen, Xin1,2; Tian, Mei1; Meng, Xueping1; Liu, Huilian3; Cheng, Hanliang1; Zhu, Changbao1; Zhao, Fangqing2; Shen, X (reprint author), Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China. | |
| 2012-09-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
 |
| ISSN | 1744-117X |
| 卷号 | 7期号:3页码:248-253 |
| 文章类型 | Article |
| 摘要 | Next-generation sequencing (NGS) has proven a valuable platform for fast and easy obtaining of large numbers of sequences at relatively low cost. In this study we use shot-gun sequencing method on Illumina HiSeq 2000, to obtain enough sequences for the assembly of the bryozoan Membranipora grandicella (Bryozoa: Cheilostomatida) mitochondrial genome, which is the first representative of the suborder Malacostegina. The complete mitochondrial genome is 15,861 bp in length, which is relatively larger than other studied bryozoans. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs and 20 transfer RNAs. To investigate the phylogenetic position and the inner relationships of the phylum Biyozoa, phylogenetic trees were constructed with amino acid sequences of 11 PCGs from 30 metazoans. Two superclades of protostomes, namely Lophotrochozoa and Ecdysozoa, are recovered as monophyletic with strong support in both ML and Bayesian analyses. Somewhat to surprise, Bryozoa appears as the sister group of Chaetognatha with moderate or high support. The relationship among five bryozoans is Tubulipora flabellaris + (M. grandicella + (Flustrellidra hispida + (Bugula neritina + Watersipora subtorquata))), which supports for the view that Cheilostomatida is not a natural, monophyletic clade. NGS proved to be a quick and easy method for sequencing a complete mitochondrial genome. (C) 2012 Elsevier Inc. All rights reserved. |
| 关键词 | Bryozoa Cheilostomatida Next-generation Sequencing Molecular Phylogeny Mitochondrial Genome |
| 学科领域 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| DOI | 10.1016/j.cbd.2012.03.003 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308115900003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12532 |
| 专题 | 海洋生物分类与系统演化实验室 |
| 通讯作者 | Shen, X (reprint author), Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China. |
| 作者单位 | 1.Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Xin,Tian, Mei,Meng, Xueping,et al. Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2012,7(3):248-253. |
| APA | Shen, Xin.,Tian, Mei.,Meng, Xueping.,Liu, Huilian.,Cheng, Hanliang.,...&Shen, X .(2012).Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,7(3),248-253. |
| MLA | Shen, Xin,et al."Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 7.3(2012):248-253. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Complete mitochondri（753KB） | 限制开放 | 使用许可 | 浏览 | |||
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