Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay

doi:10.1016/j.bios.2011.12.033

	Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay
	Wan, Yi 1,2; Qi, Peng1,2 ; Zhang, Dun 1; Wu, Jiajia1,2 ; Wang, Yi1 ; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-03-15
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	33 期号:1 页码:69-74
文章类型	Article
摘要	Nanomaterial-based enzyme-linked immunosorbent assay (ELISA) with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring. However, this goal is not yet achieved. A design for a hybrid MnO2 nanowire-ELISA using the sandwich assay format, which provides quantitative binding information for both a specific antibody and the pathogen, sulfate-reducing bacteria, and detects pathogen concentration, is presented. 3,3',5,5'-Tetramethylbenzidine was used as the substrate and was allowed to react with the MnO2 nanowires without H2O2 in the reaction system. The kinetic parameters were measured with the system acting as a catalytic biosensor. The effectiveness of the MnO2 nanowire-based biosensor was demonstrated by its sensitive detection of the pathogen. (C) 2011 Elsevier B.V. All rights reserved.
关键词	Enzyme-linked Immunosorbent Assay Manganese Oxide Bacteria Detection Nanomaterials Enzyme Mimetics
学科领域	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
DOI	10.1016/j.bios.2011.12.033
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000301699800011
引用统计	被引频次：152[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12474
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wan, Yi,Qi, Peng,Zhang, Dun,et al. Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay[J]. BIOSENSORS & BIOELECTRONICS,2012,33(1):69-74.
APA	Wan, Yi,Qi, Peng,Zhang, Dun,Wu, Jiajia,Wang, Yi,&Zhang, D .(2012).Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay.BIOSENSORS & BIOELECTRONICS,33(1),69-74.
MLA	Wan, Yi,et al."Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay".BIOSENSORS & BIOELECTRONICS 33.1(2012):69-74.

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