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Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay | |
Wan, Yi1,2; Qi, Peng1,2; Zhang, Dun1; Wu, Jiajia1,2; Wang, Yi1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
2012-03-15 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
ISSN | 0956-5663 |
卷号 | 33期号:1页码:69-74 |
文章类型 | Article |
摘要 | Nanomaterial-based enzyme-linked immunosorbent assay (ELISA) with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring. However, this goal is not yet achieved. A design for a hybrid MnO2 nanowire-ELISA using the sandwich assay format, which provides quantitative binding information for both a specific antibody and the pathogen, sulfate-reducing bacteria, and detects pathogen concentration, is presented. 3,3',5,5'-Tetramethylbenzidine was used as the substrate and was allowed to react with the MnO2 nanowires without H2O2 in the reaction system. The kinetic parameters were measured with the system acting as a catalytic biosensor. The effectiveness of the MnO2 nanowire-based biosensor was demonstrated by its sensitive detection of the pathogen. (C) 2011 Elsevier B.V. All rights reserved. |
关键词 | Enzyme-linked Immunosorbent Assay Manganese Oxide Bacteria Detection Nanomaterials Enzyme Mimetics |
学科领域 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
DOI | 10.1016/j.bios.2011.12.033 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000301699800011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/12474 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Wan, Yi,Qi, Peng,Zhang, Dun,et al. Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay[J]. BIOSENSORS & BIOELECTRONICS,2012,33(1):69-74. |
APA | Wan, Yi,Qi, Peng,Zhang, Dun,Wu, Jiajia,Wang, Yi,&Zhang, D .(2012).Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay.BIOSENSORS & BIOELECTRONICS,33(1),69-74. |
MLA | Wan, Yi,et al."Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay".BIOSENSORS & BIOELECTRONICS 33.1(2012):69-74. |
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