Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum | |
| Tang Xianghai2; Yu Rencheng1; Zhou Mingjiang1; Yu Zhigang2; Yu, RC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China. | |
| 2012-03-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 卷号 | 30期号:2页码:256-263 |
| 文章类型 | Article |
| 摘要 | The dinoflagellate Alexandrium minutum is often associated with harmful algal blooms (HABs). This species consists of many strains that differ in their ability to produce toxins but have similar morphology, making identification difficult. In this study, species-specific rRNA probes were designed for whole-cell fluorescence in situ hybridization (FISH) to distinguish A. minutum from two phylogenetic clades. We acquired the complete SSU to LSU rDNA sequences (GenBank accession numbers JF906989-JF906999) of 11 Alexandrium strains and used these to design rRNA targeted oligonucleotide probes. Three ribotype-specific probes, M-GC-1, M-PC-2, and M-PC-3, were designed. The former is specific for the GC clade ("Global clade") of A. minutum, the majority of which have been found non-toxic, and the latter two are specific for the PSP (paralytic shellfish poisoning)-producing PC clade ("Pacific clade"). The specificity of these three probes was confirmed by FISH. All cells in observed fields of view were fluorescently labeled when probes and target species were incubated under optimized FISH conditions. However, the accessibility of rRNA molecules in ribosomes varied among the probe binding positions. Thus, there was variation in the distribution of positive signals in labeled cells within nucleolus and cytosol (M-GC-1, M-PC-3), or just nucleolus (M-PC-2). Our results provide a methodological basis for studying the biogeography and population dynamics of A. minutum, and providing an early warning of toxic HABs. |
| 关键词 | Fluorescence In Situ Hybridization (Fish) Alexandrium Minutum Rrna Probe |
| 学科领域 | Limnology ; Oceanography |
| DOI | 10.1007/s00343-012-1142-7 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301001900009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12414 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yu, RC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang Xianghai,Yu Rencheng,Zhou Mingjiang,et al. Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(2):256-263. |
| APA | Tang Xianghai,Yu Rencheng,Zhou Mingjiang,Yu Zhigang,&Yu, RC .(2012).Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(2),256-263. |
| MLA | Tang Xianghai,et al."Application of rRNA probes and fluorescence in situ hybridization for rapid detection of the toxic dinoflagellate Alexandrium minutum".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.2(2012):256-263. |
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