| The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi | |
Wang, Shuangyan1,2; Cui, Zhaoxia1; Liu, Yuan1; Li, Qianqian1,2 ; Song, Chengwen1,2; Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-02-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 32期号:2页码:331-338 |
| 文章类型 | Article |
| 摘要 | Among all the serine proteinase inhibitor families (SPIs), the pacifastin-related inhibitor is seldom isolated. A pacifastin-related SPI named as PtPLC was identified from the swimming crab Portunus trituberculatus in this study. The full-length of PtPLC was cloned from haemocytes cDNA library by the combination of homology cloning and rapid amplification of cDNA ends (RACE). The PtPLC contained an open reading frame (ORF) of 1098 bp encoding a putative pacifastin-related precursor of 365 amino acids, a 5'-untranslated region (UTR) of 33 bp, and a 3'-UTR of 524 bp. The estimated molecular weight of mature PtPLC was 40.51 kDa and its isoelectric point was 5.04. Eight PLD domains in PtPLC shared a common characteristic of conserved array of six cysteine residues (Cys-Xaa(9-12)-Cys-Asn-Xaa-Cys-Xaa- Cys-Xaa(2-3)-Gly-Xaa(3-4)-Cys-Thr-Xaa(3)-Cys). The mRNA expression of PtPLC transcripts was highly detected in haemocytes, gill, hepatopancreas and stomach. The temporal expression levels of PtPLC transcripts in haemocytes showed different expression patterns after challenged by Gram-negative bacteria Vibrio alginolyticus, Gram-positive bacteria Micrococcus luteus and fungus Pichia pastoris. There were two peaks in the mRNA expression profile after M. luteus stimulation. And after V. alginolyticus and P. pastoris stimulation, there were three peaks in the mRNA expression profiles. These findings suggest that PtPLC is involved in the antibacterial defense mechanism of P. tritubercualtus. (C) 2011 Elsevier Ltd. All rights reserved. |
| 关键词 | Portunus Trituberculatus Pacifastin Mrna Expression Profile Immunity |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2011.11.025 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300751800012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12393 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 通讯作者 | Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,et al. The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(2):331-338. |
| APA | Wang, Shuangyan,Cui, Zhaoxia,Liu, Yuan,Li, Qianqian,Song, Chengwen,&Cui, ZX .(2012).The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi.FISH & SHELLFISH IMMUNOLOGY,32(2),331-338. |
| MLA | Wang, Shuangyan,et al."The first homolog of pacifastin-related precursor in the swimming crab (Portunus trituberculatus): Characterization and potential role in immune response to bacteria and fungi".FISH & SHELLFISH IMMUNOLOGY 32.2(2012):331-338. |
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