Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Subcellular localization of a lectin in Bryopsis hypnoides (Bryopsidales, Chlorophyceae) and its expression during cell organellar aggregation | |
| Xu, Meiling2; Lu, Fang1,3; Peng, Guang1; Niu, Jianfeng1; Wang, Guangce1,2; Niu, JF (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China. | |
| 2012-05-01 | |
| 发表期刊 | PHYCOLOGIA
 |
| ISSN | 0031-8884 |
| 卷号 | 51期号:3页码:340-346 |
| 文章类型 | Article |
| 摘要 | The protoplasm of giant-celled Bryopsis species is totipotent. When protoplasm is extruded from the fresh alga into seawater, the organelles - including protoplasts, nucleus and other cell contents can aggregate and reconstitute a whole cell, even regenerate into a mature individual. It is believed that a lectin carbohydrate complementary system is involved in these surprising processes. To address this issue, a polyclonal antibody against the lectin in Bryopsis hypnoides was prepared and used to evaluate the lectin's role in organellar agglutination. Results showed that the agglutination of cell organelles was blocked completely by the antibody. Real-time quantitative polymerase chain reaction of this lectin gene (EU410470.1) demonstrated that it was down-regulated, with expression reaching the lowest level at 6 h and returning to maximal level at 12 11 after protoplasm release. Western blotting revealed that a 43-kDa protein appeared at 3 h after protoplasm release, and then disappeared at 6 h. Immunogold localization indicated that the lectin was located in both chloroplast and cytoplasm. These results indicate that lectin plays an indispensable role in agglutination of the cell organelles and the regeneration of this giant-celled alga. |
| 关键词 | Aggregation Bryopsis Hypnoides Lamouroux Immunogold Labelling Lectin Real-time Quantitative Pcr Western Blotting |
| 学科领域 | Plant Sciences ; Marine & Freshwater Biology |
| DOI | 10.2216/10-37.1 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000304742700009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12388 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Niu, JF (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Meiling,Lu, Fang,Peng, Guang,et al. Subcellular localization of a lectin in Bryopsis hypnoides (Bryopsidales, Chlorophyceae) and its expression during cell organellar aggregation[J]. PHYCOLOGIA,2012,51(3):340-346. |
| APA | Xu, Meiling,Lu, Fang,Peng, Guang,Niu, Jianfeng,Wang, Guangce,&Niu, JF .(2012).Subcellular localization of a lectin in Bryopsis hypnoides (Bryopsidales, Chlorophyceae) and its expression during cell organellar aggregation.PHYCOLOGIA,51(3),340-346. |
| MLA | Xu, Meiling,et al."Subcellular localization of a lectin in Bryopsis hypnoides (Bryopsidales, Chlorophyceae) and its expression during cell organellar aggregation".PHYCOLOGIA 51.3(2012):340-346. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Subcellular localiza（402KB） | 限制开放 | 使用许可 | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论