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SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES
Wu, Xiaojie2; Niu, Jianfeng1; Huang, Aiyou1,3; Xu, Meiling2; Wang, Guangce1,2
2012-08-01
Source PublicationJOURNAL OF PHYCOLOGY
ISSN0022-3646
Volume48Issue:4Pages:1040-1044
AbstractAccurate gene quantification depends on the use of an appropriate internal control gene, which should be verified before its use for normalizing data. Housekeeping genes, which are expressed at relatively constant levels, are generally regarded as candidate internal control genes. To determine the ideal internal control for gene expression profiles for Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) at different life-history stages, we used absolute quantification to assess the expression levels of six housekeeping genes (18S ribosomal RNA, 30S ribosomal protein, glyceraldehyde-3-phosphate dehydrogenase, elongation factor 3, alpha-tubulin, and beta-tubulin) at the sporophyte and gametophyte stages. Housekeeping genes were selected by comparing the differences of observed copy numbers in sporophytes and in gametophytes. TubB (beta-tubulin) was found to be the optimal internal control gene, because it showed the smallest difference of gene expression. Compared with TubB, other housekeeping genes had greater variation of expression to different degrees.
Keywordabsolute quantification internal control gene life-history stage Porphyra haitanensis Rhodophyta
DOI10.1111/j.1529-8817.2012.01188.x
Language英语
Funding Project973 Project[2011CB411908] ; National Natural Science Foundation of China[30830015] ; National Natural Science Foundation of China[41176134] ; National Natural Science Foundation of China[30970302] ; National Marine Public Welfare Research Project[201105008-2] ; National Marine Public Welfare Research Project[201105023-7] ; Prospective Joint Research Project of Jiangsu Province[BY2011188]
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000307016700021
PublisherWILEY-BLACKWELL
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/12378
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Guangce
Affiliation1.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Xiaojie,Niu, Jianfeng,Huang, Aiyou,et al. SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES[J]. JOURNAL OF PHYCOLOGY,2012,48(4):1040-1044.
APA Wu, Xiaojie,Niu, Jianfeng,Huang, Aiyou,Xu, Meiling,&Wang, Guangce.(2012).SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES.JOURNAL OF PHYCOLOGY,48(4),1040-1044.
MLA Wu, Xiaojie,et al."SELECTION OF INTERNAL CONTROL GENE FOR EXPRESSION STUDIES IN PORPHYRA HAITANENSIS (RHODOPHYTA) AT DIFFERENT LIFE-HISTORY STAGES".JOURNAL OF PHYCOLOGY 48.4(2012):1040-1044.
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