IOCAS-IR  > 实验海洋生物学重点实验室
Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity
Hu, Yong-hua1; Dang, Wei1,2,3; Zhang, Min1; Sun, Li1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2012-10-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号33期号:4页码:829-834
文章类型Article
摘要Heat shock protein (Hsp) 70 is a molecular chaperone that plays an important role in protein folding and transport. In addition, Hsp70 is also involved in regulation of innate and adaptive immune response. In this study, we examined the biological activity and the immunomodulatory property of an Hsp70 homologue, PoHsp70, from Japanese flounder (Paralichthys olivaceus). Recombinant PoHsp70 purified from Escherichia coli exhibits apparent ATPase activity; however, a mutant PoHsp70, PoHsp70M, that bears mutation of the ATPase-associated domain, was completely abolished in activity. Expression of PoHsp70 was upregulated in a time-dependent manner by vaccination of flounder with a DNA vaccine, pSia10. that expresses a Streptococcus iniae antigen, Sia10. To examine whether PoHsp70 possessed any adjuvant potential, the DNA vaccine plasmids pSia10Hsp70 and pSia10Hsp70M were constructed. pSia10Hsp70 co-expresses Sia10 and PoHsp70, while pSia10Hsp70M co-expresses Sia10 and PoHsp70M. Following vaccination of flounder, production of Sia10 plus PoHsp70 and Sia10 plus PoHsp70M was detected in pSia10Hsp70- and pSia10Hsp70M-vaccinated fish respectively. At one month post-vaccination, comparable levels of serum antibodies were detected in fish vaccinated with pSia10Hsp70, pSia10Hsp70M, and pSia10. Subsequent protection analysis showed that, followings. iniae challenge, pSia10Hsp70 induced a survival rate that was significantly higher than that induced by pSia10, while pSia10Hsp70M induced a survival rate similar to that induced by pSia10. These results indicate that PoHsp70 is an effective adjuvant and that the adjuvanticity of PoHsp70 requires the intrinsic ATPase activity. (C) 2012 Elsevier Ltd. All rights reserved.
关键词Paralichthys Olivaceus Heat Shock Protein 70 Adjuvant Vaccine
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2012.07.015
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000310185800019
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12357
专题实验海洋生物学重点实验室
通讯作者Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位实验海洋生物学重点实验室
推荐引用方式
GB/T 7714
Hu, Yong-hua,Dang, Wei,Zhang, Min,et al. Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):829-834.
APA Hu, Yong-hua,Dang, Wei,Zhang, Min,Sun, Li,&Sun, L .(2012).Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity.FISH & SHELLFISH IMMUNOLOGY,33(4),829-834.
MLA Hu, Yong-hua,et al."Japanese flounder (Paralichthys olivaceus) Hsp70: Adjuvant effect and its dependence on the intrinsic ATPase activity".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):829-834.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Japanese flounder (P(576KB) 限制开放使用许可浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Hu, Yong-hua]的文章
[Dang, Wei]的文章
[Zhang, Min]的文章
百度学术
百度学术中相似的文章
[Hu, Yong-hua]的文章
[Dang, Wei]的文章
[Zhang, Min]的文章
必应学术
必应学术中相似的文章
[Hu, Yong-hua]的文章
[Dang, Wei]的文章
[Zhang, Min]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Japanese flounder (Paralichthys olivaceus) Hsp70 Adjuvant effect and its dependence on the intrinsic ATPase activity.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。