Hydrogen production of a salt tolerant strain Bacillus sp B2 from marine intertidal sludge

IOCAS-IR > 实验海洋生物学重点实验室

	Hydrogen production of a salt tolerant strain Bacillus sp B2 from marine intertidal sludge
	Liu, Hongyan; Wang, Guangce; Wang, GC (reprint author), Chinese Acad Sci CAS, Inst Oceanol, Qingdao 266071, Peoples R China.
	2012
发表期刊	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN	0959-3993
卷号	28 期号:1 页码:31-37
摘要	To isolate a salt tolerant hydrogen-producing bacterium, we used the sludge from the intertidal zone of a bathing beach in Tianjin as inoculum to enrich hydrogen-producing bacteria. The sludge was treated by heat-shock pretreatment with three different temperature (80, 100 and 121A degrees C) respectively. A hydrogen-producing bacterium was isolated from the sludge pretreated at 80A degrees C by sandwich plate technique and identified using microscopic examination and 16S rDNA gene sequence analysis. The isolated bacterium was named as Bacillus sp. B2. The present study examined the hydrogen-producing ability of Bacillus sp. B2. The strain was able to produce hydrogen over a wide range of initial pH from 5.0 to 10.0, with an optimum at pH 7.0. The level of hydrogen production was also affected by the salt concentration. Strain B2 has unique capability to adapt high salt concentration. It could produce hydrogen at the salt concentration from 4 to 60aEuro degrees. The maximum of hydrogen-producing yield of strain B2 was 1.65 +/- A 0.04 mol H(2)/mol glucose (mean +/- A SE) at an initial pH value of 7.0 in marine culture conditions. Hydrogen production under fresh culture conditions reached a higher level than that in marine ones. As a result, it is likely that Bacillus sp. B2 could be applied to biohydrogen production using both marine and fresh organic waste.
关键词	Enterobacter-aerogenes Biohydrogen Production Clostridium-butyricum Mixed Culture Waste-water Glucose Ph Fermentation Rna
学科领域	Biotechnology & Applied Microbiology
URL	查看原文
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12343
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC (reprint author), Chinese Acad Sci CAS, Inst Oceanol, Qingdao 266071, Peoples R China.
推荐引用方式 GB/T 7714	Liu, Hongyan,Wang, Guangce,Wang, GC . Hydrogen production of a salt tolerant strain Bacillus sp B2 from marine intertidal sludge[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2012,28(1):31-37.
APA	Liu, Hongyan,Wang, Guangce,&Wang, GC .(2012).Hydrogen production of a salt tolerant strain Bacillus sp B2 from marine intertidal sludge.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,28(1),31-37.
MLA	Liu, Hongyan,et al."Hydrogen production of a salt tolerant strain Bacillus sp B2 from marine intertidal sludge".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 28.1(2012):31-37.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Hydrogen production （271KB）			限制开放	使用许可	浏览