| First report of two thioredoxin homologues in crustaceans: Molecular characterization, genomic organization and expression pattern in swimming crab Portunus trituberculatus | |
Song, Chengwen1,2 ; Cui, Zhaoxia1; Liu, Yuan1; Wang, Shuangyan1,2; Li, Qianqian1,2; Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-05-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 32期号:5页码:855-861 |
| 文章类型 | Article |
| 摘要 | Previously, we had reported two homologues of the thioredoxin (Trx) super-family (PtTrx1 and PtTrx2) identified from eyestalk and haemocytes cDNA library of swimming crab Portunus trituberculatus, respectively. It was the first report of two thioredoxin homologues from the same crustacean species. Here, we focused on the molecular characterization, genomic organization and expression pattern of PtTrx1 and PtTrx2. The full-length cDNA sequences of PtTrx1 and PtTrx2 were 739 and 1300 bp, encoding 105 and 133 amino acids, respectively. They both had a conserved CGPC active site and highly similar tertiary structures, which containing four beta-sheets and four alpha-helixes. Specifically, PtTrx2 was encoded by a nuclear gene and its cellular localization was targeted to mitochondria by an N-terminal mitochondrial pre-sequence. Sequence analysis revealed PtTrx1 and PtTrx2 were encoded by different genomic locus. As the first analyzed genomic structure of PtTrxs in crustaceans, two introns with microsatellites were found in the open reading frame region of these genes. Quantitative real-time PCR analysis revealed the mRNA expression of PtTrx1 transcripts were mainly detected in gill, while, PtTrx2 in eyestalk and gill. The temporal expression levels of PtTrxs transcripts in haemocytes showed different expression patterns after challenge with Vibrio alginolyticus, Micrococcus luteus and Pichia pastoris. These results together indicate that PtTrxs should be involved in the responses to pathogen challenge of P. trituberculatus. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Portunus Trituberculatus Thioredoxin Genomic Structure Gene Expression |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2012.02.013 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302667200028 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12329 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 通讯作者 | Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Song, Chengwen,Cui, Zhaoxia,Liu, Yuan,et al. First report of two thioredoxin homologues in crustaceans: Molecular characterization, genomic organization and expression pattern in swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):855-861. |
| APA | Song, Chengwen,Cui, Zhaoxia,Liu, Yuan,Wang, Shuangyan,Li, Qianqian,&Cui, ZX .(2012).First report of two thioredoxin homologues in crustaceans: Molecular characterization, genomic organization and expression pattern in swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,32(5),855-861. |
| MLA | Song, Chengwen,et al."First report of two thioredoxin homologues in crustaceans: Molecular characterization, genomic organization and expression pattern in swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):855-861. |
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