Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

doi:10.1007/s00343-012-1195-7

IOCAS-IR > 实验海洋生物学重点实验室

	Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
	Wang Dongdong 1,2; Li Fuhua 1; Li Shihao 1,2; Wen Rong 1,2; Xiang Jianhai1 ; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2012-07-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	30 期号:4 页码:611-619
文章类型	Article
摘要	Antimicrobial peptides (AMPs), as key immune effectors, play important roles in the innate immune system of invertebrates. Different types of AMPs, including Penaeidin, Crustin, ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however, systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study, we analyzed the expression profiles of AMPs in the Chinese shrimps, Fenneropenaeus chinensis, simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive, G(+)) or Vibrio anguillarium (Gram-negative, G(-)). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium, and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore, the expression of these AMPs showed temporal expression profiles, suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.
关键词	Antimicrobial Peptides (Amps) Expression Profiles Bacteria Relish Rna Interference
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-012-1195-7
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000305906700013
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12327
专题	实验海洋生物学重点实验室
通讯作者	Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang Dongdong,Li Fuhua,Li Shihao,et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(4):611-619.
APA	Wang Dongdong,Li Fuhua,Li Shihao,Wen Rong,Xiang Jianhai,&Li, FH .(2012).Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(4),611-619.
MLA	Wang Dongdong,et al."Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.4(2012):611-619.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Expression profiles （449KB）			限制开放	使用许可	浏览