Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta) | |
| Xuan Jinsong1,4; Feng Yanbin1; Weng Manli1; Zhao Ge1; Shi Jinfeng1; Yao Jianting2; Wang Xiuliang2; Guo Baotai3; Qiao Lixian3; Duan Delin2; Wang Bin1; Wang, B (reprint author), Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China. | |
| 2012-11-01 | |
| 发表期刊 | ACTA OCEANOLOGICA SINICA
 |
| ISSN | 0253-505X |
| 卷号 | 31期号:6页码:139-148 |
| 文章类型 | Article |
| 摘要 | A high quality cDNA library was constructed from the brown alga Laminaria japonica, with the titer of 1.2x10(5) pfu/ml. The average insert size of the cDNA library is about 1.6 kb. From the cDNA library, 591 cDNA clones were randomly selected and sequenced. As a result, 574 EST (expressed sequence tag) sequences were generated. All of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to CX943198. The cDNA library was screened with a alpha-(32)p labeled 453 bp TPS gene probe, which is a partial sequence yielded from Porphyra yezoensis. Four positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of L. japonica TPS cDNA sequence. After PCR amplification, sequencing and assembling, the entire ORF (open reading frame) sequence of the TPS gene was obtained, which was named LjTPS. LjTPS encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 Daltons. The LjTPS gene was successfully expressed in E. coli and rice. The LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the TPS gene function and mechanism to stress tolerance. |
| 关键词 | Cdna Library Comparative Study Expressed Sequence Tag (Est) Analysis Laminaria Japonica Trehalose-6-phosphate Synthase (Tps) Tps Gene |
| 学科领域 | Oceanography |
| DOI | 10.1007/s13131-012-0260-6 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312494000014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12326 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, B (reprint author), Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, State Key Lab Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 4.Univ Sci & Technol Beijing, Dept Biol Sci & Engn, Sch Chem & Biol Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xuan Jinsong,Feng Yanbin,Weng Manli,et al. Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)[J]. ACTA OCEANOLOGICA SINICA,2012,31(6):139-148. |
| APA | Xuan Jinsong.,Feng Yanbin.,Weng Manli.,Zhao Ge.,Shi Jinfeng.,...&Wang, B .(2012).Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta).ACTA OCEANOLOGICA SINICA,31(6),139-148. |
| MLA | Xuan Jinsong,et al."Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)".ACTA OCEANOLOGICA SINICA 31.6(2012):139-148. |
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| Expressed sequence t（809KB） | 限制开放 | 使用许可 | 浏览 | |||
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