EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica

doi:10.1007/s10811-012-9807-3

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	EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica
	Liu, Fuli 1,2; Wang, Feijiu 1; Duan, Delin 2; Wang, FJ (reprint author), Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China.
	2012-06-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	24 期号:3 页码:501-505
文章类型	Article
摘要	The public availability of numerous expressed sequence tag (EST) enables EST-based SSR (simple sequence repeat) markers to be widely used for genetics and breeding studies. In the present study, EST-SSR markers were developed from ESTs of and were transferred to the non-congeneric species . Among the 2,668 non-redundant ESTs, 83 (3.1%) ESTs containing SSR were identified totally, with an average of one SSR per 13.6 kb. Analysis of SSR motifs revealed that the trinucleotide and tetranucleotide were major motifs, accounted for 44.58% and 16.87%, respectively. Based on the 83 ESTs containing SSR, we designed 45 pairs of primers in the flanking regions of the SSR, of which 13 pairs showed polymorphism in a wild population, and the mean alleles per locus was 3.6 (ranging from 2 to 6). The observed (Ho) and expected (He) heterozygosities of these EST-SSRs were 0.234-0.632 and 0.260-0.635, respectively. All loci were in Hardy-Weinberg equilibrium in the wild population and no linkage disequilibrium was detected among loci. The obtained EST-SSR markers can facilitate and promote related research such as ecological investigation, genetic diversity assessment and breeding practice of as well.
关键词	Saccharina Japonica Laminaria Digitata Est Ssr Phaeophyta
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-012-9807-3
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000304187100024
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12324
专题	实验海洋生物学重点实验室
通讯作者	Wang, FJ (reprint author), Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Fuli,Wang, Feijiu,Duan, Delin,et al. EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2012,24(3):501-505.
APA	Liu, Fuli,Wang, Feijiu,Duan, Delin,&Wang, FJ .(2012).EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica.JOURNAL OF APPLIED PHYCOLOGY,24(3),501-505.
MLA	Liu, Fuli,et al."EST-SSR markers derived from and its Laminaria digitata transferable application in Saccharina japonica".JOURNAL OF APPLIED PHYCOLOGY 24.3(2012):501-505.

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