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Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential
Jin, Ren-ping1,2; Hu, Yong-hua1; Sun, Bo-guang1; Zhang, Xiao-hua2; Sun, Li1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2012-09-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号33期号:3页码:514-521
文章类型Article
摘要Bacterial sialidases are a group of glycohydrolases that are known to play an important role in invasion of host cells and tissues. In this study, we examined in a model of Japanese flounder (Paralichthys olivaceus) the potential function of NanA, a sialidase from the fish pathogen Edwardsiella tarda. NanA is composed of 670 residues and shares low sequence identities with known bacterial sialidases. In silico analysis indicated that NanA possesses a sialidase domain and an autotransporter domain, the former containing five Asp-boxes, a RIP motif, and the conserved catalytic site of bacterial sialidases. Purified recombinant NanA (rNanA) corresponding to the sialidase domain exhibited glycohydrolase activity against sialic acid substrate in a manner that is pH and temperature dependent. Immunofluorescence microscopy showed binding of anti-rNanA antibodies to E. tarda, suggesting that NanA was localized on cell surface. Mutation of nanA caused drastic attenuation in the ability of E. tarda to disseminate into and colonize fish tissues and to induce mortality in infected fish. Likewise, cellular study showed that the nanA mutant was significantly impaired in the infectivity against cultured flounder cells. Immunoprotective analysis showed that rNanA in the form of a subunit vaccine conferred effective protection upon flounder against lethal E. tarda challenge. rNanA vaccination induced the production of specific serum antibodies, which enhanced complement-mediated bactericidal activity and reduced infection of E. tarda into flounder cells. Together these results indicate that NanA plays an important role in the pathogenesis of E tarda and may be exploited for the control of E. tarda infection in aquaculture. (C) 2012 Elsevier Ltd. All rights reserved.
关键词Neuraminidase Edwardsiella Tarda Virulence Vaccine
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2012.06.002
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000308057700006
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12316
专题实验海洋生物学重点实验室
通讯作者Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Ocean Univ China, Dept Marine Biol, Qingdao, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Jin, Ren-ping,Hu, Yong-hua,Sun, Bo-guang,et al. Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):514-521.
APA Jin, Ren-ping,Hu, Yong-hua,Sun, Bo-guang,Zhang, Xiao-hua,Sun, Li,&Sun, L .(2012).Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential.FISH & SHELLFISH IMMUNOLOGY,33(3),514-521.
MLA Jin, Ren-ping,et al."Edwardsiella tarda sialidase: Pathogenicity involvement and vaccine potential".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):514-521.
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