Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity

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	Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity
	Zhang, Min; Sun, Li; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012
发表期刊	POLISH JOURNAL OF MICROBIOLOGY
ISSN	1733-1331
卷号	61 期号:4 页码:263-271
文章类型	Article
摘要	Edwardsiella ictaluri is a Gram-negative bacterium and the causative agent of enteric septicemia of catfish. In this study, we examined the expression and function of the LuxS from a pathogenic E. ictaluri strain, J901. J901 was found to produce autoinducer 2 (AI-2) activity that maximized at mid-logarithmic phase and was enhanced by glucose and repressed by high temperature. Consistently, a luxS gene (luxS(E1)) was identified in J901, whose expression was regulated by cell density, glucose, and temperature in a manner similar to that observed with AI-2 activity. Further analysis showed that LuxS(E1) is a biologically active AI-2 synthase that was able to complement the luxS-defective phenotype of Escherichia coli DH5 alpha. To examine the functional importance of LuxS(Ei), a genetically modified variant of J901, J901Ri, was constructed, in which luxS(E1) expression was blocked by RNA interference. Compared to the wild type, J901Ri was (i) reduced in AI-2 activity to a level of 59% of that of the wild type; (ii) impaired in both planktonic and biofilm growth; (iii) significantly attenuated in the ability to infect cultured fish cells and to cause mortality in infected fish; (iv) unable to induce the expression of certain virulence-associated genes. Addition of exogenous AI-2 failed to rescue the growth defect of J901Ri as free-living cells but restored biofilm production and the expression of virulence genes to levels comparable to those of the wild type. Taken together, these results indicate that LuxS(E1) is a functional AI-2 synthase that is required for optimal cellular growth and host infection.
关键词	Edwardsiella Ictaluri Ai-2 Activity Rna Interference Quorum Sensing Virulence
学科领域	Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000317088300004
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12313
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Min,Sun, Li,Sun, L . Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity[J]. POLISH JOURNAL OF MICROBIOLOGY,2012,61(4):263-271.
APA	Zhang, Min,Sun, Li,&Sun, L .(2012).Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity.POLISH JOURNAL OF MICROBIOLOGY,61(4),263-271.
MLA	Zhang, Min,et al."Edwardsiella ictaluri LuxS: Activity, Expression, and Involvement in Pathogenicity".POLISH JOURNAL OF MICROBIOLOGY 61.4(2012):263-271.

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