Construction and analysis of experimental DNA vaccines against megalocytivirus

doi:10.1016/j.fsi.2012.09.010

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	Construction and analysis of experimental DNA vaccines against megalocytivirus
	Zhang, Min; Hu, Yong-Hua; Xiao, Zhi-Zhong; Sun, Yun; Sun, Li; Sun, L (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-11-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	33 期号:5 页码:1192-1198
文章类型	Article
摘要	Iridoviruses are large double-stranded DNA viruses with icosahedral capsid. The Iridoviridae family contains five genera, one of which is Megalocytivirus. Megalocytivirus has emerged in recent years as an important pathogen to a wide range of marine and freshwater fish. In this study, we aimed at developing effective genetic vaccines against megalocytivirus affecting farmed fish in China. For this purpose, we constructed seven DNA vaccines based on seven genes of rock bream iridovirus isolate 1 from China (RBIV-C1), a megalocytivirus with a host range that includes Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). The protective potentials of these vaccines were examined in a turbot model. The results showed that after vaccination via intramuscular injection, the vaccine plasmids were distributed in spleen, kidney, muscle, and liver, and transcription of the vaccine genes and production of the vaccine proteins were detected in these tissues. Following challenge with a lethal-dose of RBIV-C1, fish vaccinated with four of the seven DNA vaccines exhibited significantly higher levels of survival compared to control fish. Of these four protective DNA vaccines, pCN86, which is a plasmid that expresses an 86-residue viral protein, induced the highest protection. Immunological analysis showed that pCN86 was able to (i) stimulate the respiratory burst of head kidney macrophages at 14 d, 21 d, and 28 d post-vaccination, (ii) upregulate the expression of immune relevant genes involved in innate and adaptive immunity, and (iii) induce production of serum antibodies that, when incubated with RBIV-C1 before infection, significantly reduced viral loads in kidney and spleen following viral infection of turbot. Taken together, these results indicate that pCN86 is an effective DNA vaccine that may be used in the control of megalocytivirus-associated diseases in aquaculture. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Megalocytivirus Dna Vaccine Scophthalmus Maximus Immune Response
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.09.010
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000311191700015
引用统计	被引频次：47[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12305
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Min,Hu, Yong-Hua,Xiao, Zhi-Zhong,et al. Construction and analysis of experimental DNA vaccines against megalocytivirus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(5):1192-1198.
APA	Zhang, Min,Hu, Yong-Hua,Xiao, Zhi-Zhong,Sun, Yun,Sun, Li,&Sun, L .(2012).Construction and analysis of experimental DNA vaccines against megalocytivirus.FISH & SHELLFISH IMMUNOLOGY,33(5),1192-1198.
MLA	Zhang, Min,et al."Construction and analysis of experimental DNA vaccines against megalocytivirus".FISH & SHELLFISH IMMUNOLOGY 33.5(2012):1192-1198.

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