Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China | |
| Zhang, Min; Xiao, Zhi-zhong; Hu, Yong-hua; Sun, Li; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2012-03-01 | |
| 发表期刊 | AQUACULTURE RESEARCH
 |
| ISSN | 1355-557X |
| 卷号 | 43期号:4页码:556-564 |
| 文章类型 | Article |
| 摘要 | Megalocytivirus belongs to the Iridoviridae family and is known to affect finfish. Megalocytivirus epizootics have been reported to occur in several cultured fish species in China; however, no megalocytivirus associated with rock bream, Oplegnathus fasciatus (Temminck & Schlege), has been documented. In this study, we characterized for the first time a megalocytivirus, rock bream iridovirus (RBIV)-C1, detected in cultured rock bream in a fish farm in China that had been inflicted with a high-mortality disease outbreak. Sequence analysis of three conserved genes showed that RBIV-C1 shares over 90% overall identities with a number of known megalocytiviruses. Electron microscopic examination revealed RBIV-C1 as hexagonal particles similar to those reported for megalocytiviruses. In vivo infection study indicated that, following inoculation into rock bream, RBIV-C1 induced 100% mortality and upregulated the expression of Mx, IL-1 beta and IL-8. The infected fish exhibited pathological signs similar to those observed in naturally diseased fish. Furthermore, studies in a turbot (Scophthalmus maximus L.) model indicated that RBIV-C1 induced acute infection in turbot that led to 100% mortality. These results indicate that RBIV-C1 is highly virulent to rock bream as well as turbot and that RBIV-C1 is closely related to a number of previously reported megalocytivirus and likely a genetic variant of the latter. |
| 关键词 | Iridovirus Megalocytivirus Oplegnathus Fasciatus (Temminck & Schlege) Scophthalmus Maximus l |
| 学科领域 | Fisheries |
| DOI | 10.1111/j.1365-2109.2011.02861.x |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301226600009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12296 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Xiao, Zhi-zhong,Hu, Yong-hua,et al. Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China[J]. AQUACULTURE RESEARCH,2012,43(4):556-564. |
| APA | Zhang, Min,Xiao, Zhi-zhong,Hu, Yong-hua,Sun, Li,&Sun, L .(2012).Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China.AQUACULTURE RESEARCH,43(4),556-564. |
| MLA | Zhang, Min,et al."Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China".AQUACULTURE RESEARCH 43.4(2012):556-564. |
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