Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis

doi:10.1016/j.cbpb.2011.09.008

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	Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis
	Chen, Ling 1,2; Sun, Li 1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	161 期号:1 页码:54-59
文章类型	Article
摘要	Cathepsin B (EC 3.4.22.1) is a member of the papain family cysteine protease and in mammals is known to be involved in protein degradation and other biological functions. However, very little is known about the function of cathepsin B in fish. In this study, we identified and analyzed a cathepsin B homologue (CsCatB) from tongue sole (Cynoglossus semilaevis, Pleuronectiformes), an economic fish species cultured in China. CsCatB is composed of 322 amino acid residues and shares 70-81.3% overall sequence identities with its counterpart in teleosts and humans. CsCatB possesses typical cathepsin B structural features including the propeptide region and the papain family cysteine protease domain, the latter containing the four catalytic residues (Q101, C107, H277, and N297) that are conserved in lower and higher vertebrates. Quantitative real time RT-PCR analysis showed that CsCatB expression occurred in multiple tissues and was positively regulated by bacterial infection and by immunization with a subunit vaccine. Recombinant CsCatB purified from Escherichia colt exhibited apparent protease activity, which was optimal at 35 degrees C and pH 5.5. In contrast, a mutant CsCatB bearing glutamic acid substitution at H277 was dramatically reduced in proteolytic activity. These results indicate that CsCatB is a biologically active protease that is likely to be involved in host immune response during bacterial infection and vaccination. (C) 2011 Elsevier Inc. All rights reserved.
关键词	Cathepsin b Teleost Fish Cynoglossus Semilaevis Cysteine Protease
学科领域	Biochemistry & Molecular Biology ; Zoology
DOI	10.1016/j.cbpb.2011.09.008
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000297833700006
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12292
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen, Ling,Sun, Li,Sun, L . Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2012,161(1):54-59.
APA	Chen, Ling,Sun, Li,&Sun, L .(2012).Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,161(1),54-59.
MLA	Chen, Ling,et al."Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 161.1(2012):54-59.

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