Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus

doi:10.1007/s10499-011-9493-1

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	Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus
	Wang, Hongxia 1; Cui, Zhaoxia 1; Wu, Danhua 1,2; Guo, Enmian 1,3; Liu, Yuan 1,4; Wang, Chunlin 2; Su, Xiurong 2; Li, Taiwu 2; Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-08-01
发表期刊	AQUACULTURE INTERNATIONAL
ISSN	0967-6120
卷号	20 期号:4 页码:649-656
文章类型	Article
摘要	Microsatellites have been a powerful genetic tool in determining pedigree relationships among individuals. A microsatellite marker suite can be used to reliably assign parentage in the Portunus trituberculatus selective breeding programs. Thirty published microsatellites were screened for their suitability to be included in a DNA parentage marker suite. Nineteen microsatellite markers that exhibited significant stutter, poor allele resolution, null allele, and significant deviation from Mendelian expectations were eliminated from the marker suite, and the left eleven markers were ranked by PIC. Six most polymorphic markers with mean He = 0.928 and PIC = 0.901 from the original eleven were further assessed for the efficiency of pedigree identification. Total predicted power of the markers to exclude false parents was higher than 99%. Both simulations and real data analysis confirmed the high statistical power of six microsatellites suite in parentage exclusion, which permit us to trace back all offspring to single pairs of parents.
关键词	Portunus Trituberculatus Microsatellite Dna Parentage Analyses Selective Breeding
学科领域	Fisheries
DOI	10.1007/s10499-011-9493-1
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000306283000004
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12285
专题	实验海洋生物学重点实验室
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, Inst Oceanol, EMBL, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 3.Qingdao Agr Univ, Qingdao 266109, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Hongxia,Cui, Zhaoxia,Wu, Danhua,et al. Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus[J]. AQUACULTURE INTERNATIONAL,2012,20(4):649-656.
APA	Wang, Hongxia.,Cui, Zhaoxia.,Wu, Danhua.,Guo, Enmian.,Liu, Yuan.,...&Cui, ZX .(2012).Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus.AQUACULTURE INTERNATIONAL,20(4),649-656.
MLA	Wang, Hongxia,et al."Application of microsatellite DNA parentage markers in the swimming crab Portunus trituberculatus".AQUACULTURE INTERNATIONAL 20.4(2012):649-656.

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