C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine

doi:10.3354/dao02544

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	C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine
	Yu, Lan-Ping 1,2; Hu, Yong-Hua 1; Sun, Bo-Guang1 ; Sun, Li 1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012-12-03
发表期刊	DISEASES OF AQUATIC ORGANISMS
ISSN	0177-5103
卷号	102 期号:1 页码:33-42
文章类型	Article
摘要	Vibrio anguillarum, a Gram-negative bacterial pathogen, is the causative agent of vibriosis that affects a wide range of aquatic animals. In this study, we obtained a mutant V. anguillarum, C312M, derived from the pathogenic V. anguillarum C312 by selection of rifampicin resistance. C312M was slower in growth than the wild type C312, particularly under conditions of iron depletion. Compared to C312, C312M was altered in protein production profile and exhibited a dramatically increased median lethal dose. Safety analysis showed that C312M was stable in virulence in the absence of selective pressure. To examine the potential of C312M as a live attenuated vaccine, Japanese flounder Paralichthys olivaceus were vaccinated with C312M via oral, immersion, and oral plus immersion routes. Microbiological analysis showed that C312M was recovered from the gut, liver, kidney, and spleen of the vaccinated fish in 1 to 14 d post-vaccination. When the fish were challenged with C312 at 1 mo post-vaccination, C312M-vaccinated fish exhibited relative percent survival rates of 60 to 84%. Comparable protection was observed when the fish were challenged with a heterologous V. anguillarum strain. Further analysis showed that C312M-vaccinated fish produced specific serum antibodies which enhanced serum bactericidal activity in a manner that is probably complement-dependent. These results indicate that C312M is highly attenuated in virulence but still retains residual infectivity, and that C312M is an effective vaccine when delivered alive via immersion and oral feeding.
关键词	Vibrio Anguillarum Attenuated Vaccine Rifampicin Resistance Immersion Oral Feeding
学科领域	Fisheries ; Veterinary Sciences
DOI	10.3354/dao02544
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000311967600004
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12278
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yu, Lan-Ping,Hu, Yong-Hua,Sun, Bo-Guang,et al. C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine[J]. DISEASES OF AQUATIC ORGANISMS,2012,102(1):33-42.
APA	Yu, Lan-Ping,Hu, Yong-Hua,Sun, Bo-Guang,Sun, Li,&Sun, L .(2012).C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine.DISEASES OF AQUATIC ORGANISMS,102(1),33-42.
MLA	Yu, Lan-Ping,et al."C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine".DISEASES OF AQUATIC ORGANISMS 102.1(2012):33-42.

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