| A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs | |
Liu, Yuan1,2; Cui, Zhaoxia1; Li, Xihong1,2; Song, Chengwen1,2 ; Li, Qianqian1,2; Wang, Shuangyan1,2; Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
| |
| 2012-05-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| 卷号 | 32期号:5页码:724-731 |
| 文章类型 | Article |
| 摘要 | Anti-lipopolysaccharide factors (ALFs) are the potent antimicrobial peptides that can bind and neutralize lipopolysaccharide (LPS). In this study, a new isoform of the ALF homologs (PtALF4) was cloned from eyestalk cDNA library of swimming crab Portunus trituberculatus. PtALF4 shared lower amino acid similarity with other ALFs, yet it contained the conserved LPS-binding domain and was clearly member of the ALF family. The genomic sequence of PtALF4 consisted of three exons interrupted by two introns. Several tandem repeats were found in both introns. Unlike most ALFs expressed in hemocytes, PtALF4 transcript was predominantly detected in eyestalk. After challenge with Vibrio alginolyticus, the temporal expression level of PtALF4 transcript in hemocytes showed a clear time-dependent response expression pattern with two significant peaks. The recombinant proteins of PtALF1, PtALF3 and PtALF4 revealed different antimicrobial activities against bacteria or fungus. These results together suggest that PtALF isoforms might be potent immune effectors to provide multiple protective functions against invading bacteria or fungus in P. trituberculatus. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Portunus Trituberculatus Anti-lipopolysaccharide Factor Expression Analysis Antimicrobial Activity |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2012.01.021 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302667200013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12260 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 通讯作者 | Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Yuan,Cui, Zhaoxia,Li, Xihong,et al. A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):724-731. |
| APA | Liu, Yuan.,Cui, Zhaoxia.,Li, Xihong.,Song, Chengwen.,Li, Qianqian.,...&Cui, ZX .(2012).A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs.FISH & SHELLFISH IMMUNOLOGY,32(5),724-731. |
| MLA | Liu, Yuan,et al."A new anti-lipopolysaccharide factor isoform (PtALF4) from the swimming crab Portunus trituberculatus exhibited structural and functional diversity of ALFs".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):724-731. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A new anti-lipopolys（1595KB） | 限制开放 | 使用许可 | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论