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A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis
Wen, Rong1,2; Li, Fuhua1; Xie, Yusu1; Li, Shihao1,2; Xiang, Jianhai1; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
2012
发表期刊BIOLOGY OF REPRODUCTION
ISSN0006-3363
卷号86期号:1
文章类型Article
摘要The cell apoptosis susceptibility (CAS) gene is a homolog of the yeast chromosome segregation (CSE1) gene, which functions in cell proliferation and apoptosis. In the present study, a homolog of CAS was cloned from Chinese shrimp Fenneropenaeus chinensis (FcCAS). The full-length FcCAS cDNA is 3534 bp and contains an open reading frame encoding 968 amino acids. The predicted tertiary FcCAS structure is highly similar to that of CSE1 from the yeast Saccharomyces cerevisiae. RT-PCR analysis showed that the FcCAS gene is expressed mainly in testis, ovary, stomach, lymphoid organs, gills, and hemocytes. RNA in situ hybridization showed that FcCAS transcripts were distributed mainly in the cytoplasm of oocytes. Western blot analysis showed that FcCAS could be detected only in testis and ovary, and its expression levels differed at different developmental stages of ovaries. Immunohistochemical analysis showed that FcCAS existed in both the cytoplasm and the nucleus, which suggested that FcCAS might function as a nuclear protein. No transcript was detected in the abnormally developed ovaries of triploid shrimp. Therefore, we inferred that the FcCAS gene might be one of the key genes that is closely related to ovary development in shrimp.
关键词Cas Gene Expression Invertebrates Oocyte Ovary Ovary Development Shrimp
学科领域Reproductive Biology
DOI10.1095/biolreprod.111.092635
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收录类别SCI
语种英语
WOS记录号WOS:000300548400006
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12258
专题实验海洋生物学重点实验室
通讯作者Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位中国科学院海洋研究所
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Wen, Rong,Li, Fuhua,Xie, Yusu,et al. A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis[J]. BIOLOGY OF REPRODUCTION,2012,86(1).
APA Wen, Rong,Li, Fuhua,Xie, Yusu,Li, Shihao,Xiang, Jianhai,&Li, FH .(2012).A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis.BIOLOGY OF REPRODUCTION,86(1).
MLA Wen, Rong,et al."A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis".BIOLOGY OF REPRODUCTION 86.1(2012).
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