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Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)
Lin, Fan1,2; Liu, Qinghua1; Li, Mingyou3; Li, Zhendong3; Hong, Ni3; Li, Jun1; Hong, Yunhan3; Li, J (reprint author), Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China.
2012
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
卷号8期号:6页码:882-890
文章类型Article
摘要Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised to visualize, isolate and transplant PGCs within and between species. In fish, stable PGC visualization in live embryo and individual has been limited to laboratory fish models such as medaka and zebrafish. One exception is the rainbow trout, which represents the only species with aquaculture importance and has GFP-labeled germ cells throughout development. PGCs can be transiently labeled by embryonic injection of mRNA containing green fluorescence protein gene (GFP) and 3'-untranslated region (3'-UTR) of a maternal germ gene such as vasa, nos 1, etc. Stable PGC labeling can be achieved through production of transgenic animals by some transcriptional regulatory sequences from germ genes, such as the vasa promoter and 3'-UTR. In this study, we reported the functional analyses of the red seabream vasa (Pmvas) regulatory sequences, using medaka as a model system. It was showed that injection of GFP-Pmvas3'UTR mRNA was able to label medaka PGCs during embryogenesis. Besides, we have constructed pPmvasGFP transgenic vector, and established a stable transgenic medaka line exhibiting GFP expression in germ cells including PGCs, mitotic and meiotic germ cells of both sexes, under control of the Pmvas transcriptional regulatory sequences. It is concluded that the Pmvas regulatory sequences examined in this study are sufficient for germ cell expression and labeling.
关键词Pagrus Major Pgcs Vasa Transgene Gfp
学科领域Biochemistry & Molecular Biology ; Biology
DOI10.7150/ijbs.4421
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000306062300010
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12254
专题海洋生物技术研发中心
通讯作者Li, J (reprint author), Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
第一作者单位中国科学院海洋研究所
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Lin, Fan,Liu, Qinghua,Li, Mingyou,et al. Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2012,8(6):882-890.
APA Lin, Fan.,Liu, Qinghua.,Li, Mingyou.,Li, Zhendong.,Hong, Ni.,...&Li, J .(2012).Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major).INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,8(6),882-890.
MLA Lin, Fan,et al."Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 8.6(2012):882-890.
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