Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major) | |
Lin, Fan1,2; Liu, Qinghua1; Li, Mingyou3; Li, Zhendong3; Hong, Ni3; Li, Jun1; Hong, Yunhan3; Li, J (reprint author), Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China. | |
2012 | |
发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES |
ISSN | 1449-2288 |
卷号 | 8期号:6页码:882-890 |
文章类型 | Article |
摘要 | Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised to visualize, isolate and transplant PGCs within and between species. In fish, stable PGC visualization in live embryo and individual has been limited to laboratory fish models such as medaka and zebrafish. One exception is the rainbow trout, which represents the only species with aquaculture importance and has GFP-labeled germ cells throughout development. PGCs can be transiently labeled by embryonic injection of mRNA containing green fluorescence protein gene (GFP) and 3'-untranslated region (3'-UTR) of a maternal germ gene such as vasa, nos 1, etc. Stable PGC labeling can be achieved through production of transgenic animals by some transcriptional regulatory sequences from germ genes, such as the vasa promoter and 3'-UTR. In this study, we reported the functional analyses of the red seabream vasa (Pmvas) regulatory sequences, using medaka as a model system. It was showed that injection of GFP-Pmvas3'UTR mRNA was able to label medaka PGCs during embryogenesis. Besides, we have constructed pPmvasGFP transgenic vector, and established a stable transgenic medaka line exhibiting GFP expression in germ cells including PGCs, mitotic and meiotic germ cells of both sexes, under control of the Pmvas transcriptional regulatory sequences. It is concluded that the Pmvas regulatory sequences examined in this study are sufficient for germ cell expression and labeling. |
关键词 | Pagrus Major Pgcs Vasa Transgene Gfp |
学科领域 | Biochemistry & Molecular Biology ; Biology |
DOI | 10.7150/ijbs.4421 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000306062300010 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/12254 |
专题 | 海洋生物技术研发中心 |
通讯作者 | Li, J (reprint author), Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China. |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Lin, Fan,Liu, Qinghua,Li, Mingyou,et al. Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2012,8(6):882-890. |
APA | Lin, Fan.,Liu, Qinghua.,Li, Mingyou.,Li, Zhendong.,Hong, Ni.,...&Li, J .(2012).Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major).INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,8(6),882-890. |
MLA | Lin, Fan,et al."Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 8.6(2012):882-890. |
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