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The enhancement of hydrogen photoproduction in Chlorella protothecoides exposed to nitrogen limitation and sulfur deprivation
He, Meilin1,2; Li, Ling1; Zhang, Litao1; Liu, Jianguo1; Liu, JG (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China.
2012-11-01
发表期刊INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
ISSN0360-3199
卷号37期号:22页码:16903-16915
文章类型Article
摘要H-2 photoproduction, hydrogenase activities and PSII photochemical activities in Chlorella protothecoides under sulfur (S-) or nitrogen (N-) deprivation or simultaneous N-limitation and S-deprivation were studied. C. protothecoides pre-cultured in full nutrient TAP medium containing 7 mM NH4Cl was found to produce a detectable but low level of H-2, once the cells were inoculated either in S-free or N-free medium. However, cells pre-grown in a low concentration of NH4Cl (0.35 and 0.7 mM) generated a large amount of H-2 after transfer to N-limited and S-free medium. The maximal H-2 outputs of similar to 233.7 and similar to 129.1 ml/l were obtained within 100 h in the cultures exposed to S-deprived medium containing 0.35 mM and 0.7 mM NH4Cl, with the average H-2 production rates being similar to 2.19 and similar to 1.37 ml/l/h, respectively. Our studies further indicated that N-limitation resulted in considerable starch accumulation, chlorophyll synthesis reduction, photosynthetic electron transfer block and oxygen evolving complex (OEC) injury, as well as attenuation in PSII oxygenic activity. Significant starch degradation was not observed during the H-2 photoevolution process. Attenuation of PSII O-2 evolution favored a rapid establishment of anaerobiosis for hydrogenase induction. Meanwhile, a constant high level of hydrogenase activities in C. protothecoides exposed to simultaneous N-limitation and S-deprivation were measured. Based on the above results, a possible mechanism of high H-2 photoproduction in C. protothecoides exposed to N-limitation and S-deprivation was discussed. Low net photosynthetic oxygenic rates, together with high hydrogenase activities were thought to contribute to the enhancement of H-2 photoproduction by C. protothecoides. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
关键词Hydrogen Photoproduction Chlorella Protothecoides Nitrogen Limitation Sulfur Deprivation
学科领域Chemistry, Physical ; Electrochemistry ; Energy & Fuels
DOI10.1016/j.ijhydene.2012.08.121
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000311188500018
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12249
专题海洋生物技术研发中心
通讯作者Liu, JG (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 10049, Peoples R China
第一作者单位中国科学院海洋研究所
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GB/T 7714
He, Meilin,Li, Ling,Zhang, Litao,et al. The enhancement of hydrogen photoproduction in Chlorella protothecoides exposed to nitrogen limitation and sulfur deprivation[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2012,37(22):16903-16915.
APA He, Meilin,Li, Ling,Zhang, Litao,Liu, Jianguo,&Liu, JG .(2012).The enhancement of hydrogen photoproduction in Chlorella protothecoides exposed to nitrogen limitation and sulfur deprivation.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,37(22),16903-16915.
MLA He, Meilin,et al."The enhancement of hydrogen photoproduction in Chlorella protothecoides exposed to nitrogen limitation and sulfur deprivation".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 37.22(2012):16903-16915.
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