| Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas | |
| Zhang LinLin; Li Li; Zhang GuoFan; Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. | |
| 2012-09-01 | |
| 发表期刊 | CHINESE SCIENCE BULLETIN
 |
| ISSN | 1001-6538 |
| 卷号 | 57期号:25页码:3312-3319 |
| 文章类型 | Article |
| 摘要 | Fibrinogen-related proteins (FREPs) are lectins with at least a fibrinogen-like domain. They are widespread in Mollusca and play an important role as immune pattern-recognition receptors. These diverse molecules provide interesting models for the study of the evolution of innate immunity. Although oysters are among the most studied mollusks, there have been few investigations of this gene family in oysters. This paper reports the cloning of the first oyster FREP, named CgFREP, from the Pacific oyster Crassostrea gigas. Five types of FREP (CgFREP-1 to CgFREP-5) were obtained based on a single pair of primers, confirming their high diversity. The proteins deduced from these FREPs contained 253-363 amino acids and were divided into three subfamilies based on sequence identities (cutoff point < 86%). The topological structure of the phylogenetic tree showed the five CgFREPs clustered together and were completely separated from the subgroup of FREPs obtained from Biomphalaria glabrata (BgFREPs). This implies that FREP diversity evolved before the BgFREPs branched off. An interesting aspect of the CgFREPs was their individual hypermutation. Ninety-three SNPs and five indels were characterized among CgFREPs. Some mutations (e.g. an insertion in CgFREP-5) could cause premature translation termination. Multiple alignments suggest recombination events might have occurred during exchanges between alleles, or by somatic diversification, or both. Individual sequence variability parallels the extraordinary complexity of the innate immune system, suggesting a potential capacity to recognize and eliminate many kinds of pathogens, even in a primitive system. The diversity of immune pattern-recognition receptors may offer an alternative strategy that functions in the absence of highly specific immune globulins, which only emerged in vertebrates. |
| 关键词 | Crassostrea Gigas Freps Fibrinogen-related Proteins Recombination Oysters |
| 学科领域 | Multidisciplinary Sciences |
| DOI | 10.1007/s11434-012-5155-6 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307750500009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12245 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang LinLin,Li Li,Zhang GuoFan,et al. Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3312-3319. |
| APA | Zhang LinLin,Li Li,Zhang GuoFan,&Li, L .(2012).Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas.CHINESE SCIENCE BULLETIN,57(25),3312-3319. |
| MLA | Zhang LinLin,et al."Sequence variability of fibrinogen-related proteins (FREPs) in Crassostrea gigas".CHINESE SCIENCE BULLETIN 57.25(2012):3312-3319. |
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