| Separation of chito-oligomers with several degrees of polymerization and study of their antioxidant activity | |
Li, Kecheng1,2; Xing, Ronge1 ; Liu, Song1; Li, Rongfeng1,2; Qin, Yukun1,2; Meng, Xiangtao1,2; Li, Pengcheng1; Li, PC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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| 2012-04-15 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
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| ISSN | 0144-8617 |
| 卷号 | 88期号:3页码:896-903 |
| 文章类型 | Article |
| 摘要 | Chitooligomers (COS) with degree of polymerization (DP) ranging from 2 to 12 was prepared by acid hydrolysis of chitosan and five fractions were separated from the prepared COS by CM Sephadex C-25 column. The fractions were desalted by activated charcoal extraction. The components of each fraction were analyzed by TLC, HPLC and MALDI-TOFMS. Five fractions mainly contained trimer (95.7%), tetramer and pentamer (90.9%), pentamer (85.6%), hexamer (89.5%) and heptamer to decamer (94.7%), respectively. The antioxidant activities of each fraction and COS mixture were investigated, including hydroxyl and superoxide radical scavenging activity and reducing power. The activities of the six COSs were in a dose-dependence manner and related to their DP. The COS with low DP showed better effect of scavenging hydroxyl radical and reducing power than that with high DP. In contrast, the superoxide radical scavenging activity of all the tested COSs increased with DP increasing. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Chitooligomers Separation Desalination Antioxidant Activity |
| 学科领域 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| DOI | 10.1016/j.carbpol.2012.01.033 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302196200013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12244 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Li, PC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Kecheng,Xing, Ronge,Liu, Song,et al. Separation of chito-oligomers with several degrees of polymerization and study of their antioxidant activity[J]. CARBOHYDRATE POLYMERS,2012,88(3):896-903. |
| APA | Li, Kecheng.,Xing, Ronge.,Liu, Song.,Li, Rongfeng.,Qin, Yukun.,...&Li, PC .(2012).Separation of chito-oligomers with several degrees of polymerization and study of their antioxidant activity.CARBOHYDRATE POLYMERS,88(3),896-903. |
| MLA | Li, Kecheng,et al."Separation of chito-oligomers with several degrees of polymerization and study of their antioxidant activity".CARBOHYDRATE POLYMERS 88.3(2012):896-903. |
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