Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro

doi:10.1016/j.ijbiomac.2012.01.018

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	Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro
	Meng, Xiangtao 1,2; Xing, Ronge1 ; Liu, Song 1; Yu, Huahua1 ; Li, Kecheng 1,2; Qin, Yukun 1,2; Li, Pengcheng1 ; Li, PC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2012-05-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	50 期号:4 页码:918-924
文章类型	Article
摘要	Aminoethyl modified chitosan derivatives (AEMCSs) with different molecular weight (Mw) were synthesized by grafting aminoethyl group on different molecular weight chitosans and chitooligosaccharide. FTIR, H-1 NMR, C-13 NMR, elemental analysis and potentiometric titration results showed that branched polyethylimine chitosan was synthesized. Clinical Laboratory Standard Institute (CLSI) protocols were used to determine MIC for Gram-negative strain of Escherichia coli under different pH. The antibacterial activity of the derivatives was significantly improved compared with original chitosans, with MIC values against E. coli varying from 4 to 64 mu g/mL depending on different Mw and pH. High molecular weight seems to be in favor of stronger antibacterial activity. At pH 7.4, derivatives with Mw above 27 kDa exhibited equivalent antibacterial activity (16 mu g/mL), while oligosaccharide chitosan derivative with lower Mw (similar to 1.4 kDa) showed decreased MIC of 64 mu g/mL.. The effect of pH on antibacterial activity is more complicated. An optimal pH for HAEMCS was found around 6.5 to give MIC as low as 4 mu g/mL, while higher or lower pH compromised the activity. Cell integrity assay and SEM images showed evident cell disruption, indicating membrane disruption may be one possible mechanism for antibacterial activity. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Chitosan Chitooligosaccharide Molecular Weight Ph Antibacterial Polyethylimine
学科领域	Biochemistry & Molecular Biology
DOI	10.1016/j.ijbiomac.2012.01.018
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000304230000007
引用统计	被引频次：45[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12236
专题	海洋生物技术研发中心海洋环境工程技术研究发展中心
通讯作者	Li, PC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Meng, Xiangtao,Xing, Ronge,Liu, Song,et al. Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2012,50(4):918-924.
APA	Meng, Xiangtao.,Xing, Ronge.,Liu, Song.,Yu, Huahua.,Li, Kecheng.,...&Li, PC .(2012).Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,50(4),918-924.
MLA	Meng, Xiangtao,et al."Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 50.4(2012):918-924.

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