| Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei | |
Lin, Fan1,2; Xu, Shihong1; Ma, Daoyuan1; Xiao, Zhizhong1; Zhao, Chunyan1,2 ; Xiao, Yongshuang1; Chi, Liang1,2; Liu, Qinghua1; Li, Jun1; Liu, QH (reprint author), Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-11-01 | |
| 发表期刊 | MOLECULAR REPRODUCTION AND DEVELOPMENT
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| ISSN | 1040-452X |
| 卷号 | 79期号:11页码:803-813 |
| 文章类型 | Article |
| 摘要 | Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from turbot (Scophthalmus maximus), a marine Euteleostei species, and investigated its expression patterns by RT-PCR and in situ hybridization during embryogenesis and gametogenesis to identify the germ cell lineage in this species. The deduced amino acid sequence of the isolated cDNA shared typical characteristics of Vasa protein and high identity to Vasa homologues in medaka (76.9%) and zebrafish (68.5%). The Smvas transcripts were exclusively detected in germ cells of testis and ovary, and exhibited an interesting dynamic localization pattern during oogenesis. The distribution pattern of Smvas during embyogenesis in this Euteleostei closely resembled the pattern observed in zebrafish (belonging to Osteriophysans) rather than medaka (belonging to Euteleostei). Thus, it is concluded that Smvas isolated in this study is a germ cell specific molecular marker in turbot. Furthermore, we hypothesize that Euteleostei could localize vasa mRNA by a special mode. The results not only facilitate the germ cell manipulation of the turbot, but also improve our understanding of germline development and evolution of vasa localization in teleost. Mol. Reprod. Dev. 79: 803813, 2012. (C) 2012 Wiley Periodicals, Inc. |
| 关键词 | Differential Expression Rainbow-trout Teleost Fish Cell Lineage Zebrafish Rna Generation Oogenesis Embryos Cloning |
| 学科领域 | Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology |
| DOI | 10.1002/mrd.22120 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311297000007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12227 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, QH (reprint author), Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Lin, Fan,Xu, Shihong,Ma, Daoyuan,et al. Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2012,79(11):803-813. |
| APA | Lin, Fan.,Xu, Shihong.,Ma, Daoyuan.,Xiao, Zhizhong.,Zhao, Chunyan.,...&Liu, QH .(2012).Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei.MOLECULAR REPRODUCTION AND DEVELOPMENT,79(11),803-813. |
| MLA | Lin, Fan,et al."Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei".MOLECULAR REPRODUCTION AND DEVELOPMENT 79.11(2012):803-813. |
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