Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition | |
Tong, X. H.1; Xu, S. H.2; Liu, Q. H.2; Li, J.2; Xiao, Z. Z.2; Ma, D. Y.2; Tong, XH (reprint author), Yancheng Teachers Univ, Yancheng 224002, Peoples R China. | |
2012-06-01 | |
发表期刊 | FISH PHYSIOLOGY AND BIOCHEMISTRY |
ISSN | 0920-1742 |
卷号 | 38期号:3页码:715-724 |
文章类型 | Article |
摘要 | Digestive enzyme activities were analysed in turbot () from hatching until 60 days after hatching (DAH). Trypsin sharply increased to the climax at 17 DAH and decreased until 31 DAH followed by a stable level thereafter. Amylase was determined at 4 DAH, reached the maximum value at 19 DAH and declined sharply to 39 DAH and remained at a low level thereafter, suggesting the carbohydrate component should remain at a low level in formulated diets. Pepsin was detected at 9 DAH and increased to 34 DAH and then remained at a stable level. The above results revealed pancreatic enzymes are no longer main enzymes for food digestion after the formation of functional stomach. Leucine-alanine peptidase (Leu-ala) and alkaline phosphatase (AP) and leucine aminopeptidase N (LAP) were found in newly hatched larvae. Both AP and LAP activities markedly increased to 23 DAH, decreased abruptly to 50 DAH and increased gradually to 60 DAH. Leu-ala reached the plateau from 23 to 39 DAH, followed by a decline to 46 DAH and an increase until 60 DAH. The brush border membrane (BBM)-bound enzyme activities increased from 30% at 31 DAH to 81% at 38 DAH of the total activities, indicating the maturation of intestinal tract. |
关键词 | Enzymatic Assay Intestinal Enzymes Ontogeny Pancreatic Enzymes Scophthalmus Maximus |
学科领域 | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
DOI | 10.1007/s10695-011-9553-5 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000304119100012 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/12220 |
专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 |
通讯作者 | Tong, XH (reprint author), Yancheng Teachers Univ, Yancheng 224002, Peoples R China. |
作者单位 | 1.Yancheng Teachers Univ, Yancheng 224002, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Tong, X. H.,Xu, S. H.,Liu, Q. H.,et al. Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2012,38(3):715-724. |
APA | Tong, X. H..,Xu, S. H..,Liu, Q. H..,Li, J..,Xiao, Z. Z..,...&Tong, XH .(2012).Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition.FISH PHYSIOLOGY AND BIOCHEMISTRY,38(3),715-724. |
MLA | Tong, X. H.,et al."Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition".FISH PHYSIOLOGY AND BIOCHEMISTRY 38.3(2012):715-724. |
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