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Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris
Li, Rongfeng1,2; Yu, Huahua1; Xing, Ronge1; Liu, Song1; Qing, Yukun1,2; Li, Kecheng1,2; Li, Bing1,2; Meng, Xiangtao1,2; Cui, Jinhui1,2; Li, Pengcheng1; Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
2012-06-15
发表期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
卷号899页码:86-95
文章类型Article
摘要The nematocyst proteins of jellyfish Stomolophus meleagris, a complicated mixture, contain many important bioactive molecules. In present study, to gain comprehensive insight into the protein component and search some novel bioactive molecules in the nematocyst proteins, shotgun proteomic analysis of the nematocyst proteins was carried out by nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) for the first time. Digested peptides of the nematocyst proteins were analyzed by nanoLC-MS/MS and all MS/MS spectra were then automatically searched by the SEQUEST program. A total of 181 proteins had been identified, with the molecular weight ranging from 5268.06 to 843,487.57 and the pi from 4.49 to 11.39. Bioinformatic analysis was also applied to better understand the identified proteins. In the gene ontology (GO) annotation, all the identified proteins were classified into 13.9 and 7 groups according to biological process, cellular component and molecular function, respectively. Pathways analysis of the identified proteins was conducted with 33 corresponding pathways found. On the basis of pathways analysis, we also constructed the gene network to analyze the relationship of those genes each other, which contained enzyme-enzyme relation, protein-protein interaction and gene expression interaction. (C) 2012 Elsevier B.V. All rights reserved.
关键词Jellyfish Stomolophus Meleagris Nematocyst Proteins Shotgun Nanolc-ms/ms Proteomic Analysis
学科领域Biochemical Research Methods ; Chemistry, Analytical
DOI10.1016/j.jchromb.2012.05.006
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000306247300013
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12213
专题海洋生物技术研发中心
海洋环境工程技术研究发展中心
通讯作者Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所
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Li, Rongfeng,Yu, Huahua,Xing, Ronge,et al. Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,899:86-95.
APA Li, Rongfeng.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Qing, Yukun.,...&Yu, HH .(2012).Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,899,86-95.
MLA Li, Rongfeng,et al."Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 899(2012):86-95.
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