An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas

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	An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas
	Wang, Xiaotong; Song, Xiaorui; Li, Li; Zhang, Guofan; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
	2012
发表期刊	ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
ISSN	0792-156X
卷号	64 页码:800
文章类型	Article
摘要	We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation.
关键词	Mollusc Bivalve Pacific Oyster Shell Dna Marker-assisted Selection (Mas)
学科领域	Fisheries
收录类别	SCI
语种	英语
WOS记录号	WOS:000316859200001
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12211
专题	海洋生物技术研发中心实验海洋生物学重点实验室
通讯作者	Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xiaotong,Song, Xiaorui,Li, Li,et al. An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas[J]. ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,2012,64:800.
APA	Wang, Xiaotong,Song, Xiaorui,Li, Li,Zhang, Guofan,&Zhang, GF .(2012).An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas.ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,64,800.
MLA	Wang, Xiaotong,et al."An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas".ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH 64(2012):800.

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