IOCAS-IR  > 海洋生物技术研发中心
STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS
Lin, F.1,2; Liu, Q. H.1; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.; Xiao, Y. S.; Li, J.1
2011-07-01
发表期刊CRYOLETTERS
ISSN0143-2044
卷号32期号:4页码:339-348
文章类型Article
摘要The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%-40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (< 50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was > 50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.; The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%-40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (< 50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was > 50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.
关键词Pagrus Major Dmso Permeability Capillary Electrophoresis Hatching Rate Morphology Changes
学科领域Life Sciences & Biomedicine - Other Topics ; Physiology
收录类别SCI
语种英语
WOS记录号WOS:000295308200006
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12059
专题海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Lin, F.,Liu, Q. H.,Xiao, Z. Z.,et al. STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS[J]. CRYOLETTERS,2011,32(4):339-348.
APA Lin, F..,Liu, Q. H..,Xiao, Z. Z..,Ma, D. Y..,Xu, S. H..,...&Li, J..(2011).STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS.CRYOLETTERS,32(4),339-348.
MLA Lin, F.,et al."STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS".CRYOLETTERS 32.4(2011):339-348.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Study on Permeabilit(665KB) 限制开放--浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lin, F.]的文章
[Liu, Q. H.]的文章
[Xiao, Z. Z.]的文章
百度学术
百度学术中相似的文章
[Lin, F.]的文章
[Liu, Q. H.]的文章
[Xiao, Z. Z.]的文章
必应学术
必应学术中相似的文章
[Lin, F.]的文章
[Liu, Q. H.]的文章
[Xiao, Z. Z.]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Study on Permeability of DMSO in Embryos of Red Seabream by Capillary Electrophoresis.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。