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Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein
Ding, Jianfeng1,2,3; Li, Juan1,2; Bao, Yongbo1,2; Li, Li1; Wu, Fucun1; Zhang, Guofan1
2011
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号30期号:1页码:163-172
文章类型Article
摘要A chicken-type lysozyme was obtained and characterized from the abalone Haliotis discus hannai In (HdLysC). The full-length cDNA of HdLysC was 586 bp, and it contained an open reading frame of 441 bp, encoding a 147-amino acid protein with a calculated molecular mass of 15.64 kDa and an isoelectric point of 4.87. The amino acid sequence of HdLysC possessed all conserved features critical for the fundamental structure and function of c-type lysozymes, including the two catalytic residues, Glu54 and Asp70. The genomic length of HdLysC was 2865 bp, with four exons interrupted by three introns. The genomic structure of HdLysC was more similar to vertebrates than invertebrates. Many putative transcription factor binding sites involved in the immune system and cancer were found in the promoter region of HdLysC. Quantitative real-time RT-PCR detected HdLysC expression in all examined tissues, as well as in an expression profile of abalone gills challenged with bacteria Vibrio anguillarum. HdLysC transcripts were found to be most abundantly expressed in mantles, and weakly expressed in hemocytes, and increased expression of HdLysC also observed after bacterial stimulation. HdLysC was expressed in Escherichia coli, and the recombinant protein showed bacteriolytic activity against both Gram-positive and Gram-negative bacteria. Of the Vibrio species tested, more effective activity was detected against V. anguillarum. (C) 2010 Elsevier Ltd. All rights reserved.; A chicken-type lysozyme was obtained and characterized from the abalone Haliotis discus hannai In (HdLysC). The full-length cDNA of HdLysC was 586 bp, and it contained an open reading frame of 441 bp, encoding a 147-amino acid protein with a calculated molecular mass of 15.64 kDa and an isoelectric point of 4.87. The amino acid sequence of HdLysC possessed all conserved features critical for the fundamental structure and function of c-type lysozymes, including the two catalytic residues, Glu54 and Asp70. The genomic length of HdLysC was 2865 bp, with four exons interrupted by three introns. The genomic structure of HdLysC was more similar to vertebrates than invertebrates. Many putative transcription factor binding sites involved in the immune system and cancer were found in the promoter region of HdLysC. Quantitative real-time RT-PCR detected HdLysC expression in all examined tissues, as well as in an expression profile of abalone gills challenged with bacteria Vibrio anguillarum. HdLysC transcripts were found to be most abundantly expressed in mantles, and weakly expressed in hemocytes, and increased expression of HdLysC also observed after bacterial stimulation. HdLysC was expressed in Escherichia coli, and the recombinant protein showed bacteriolytic activity against both Gram-positive and Gram-negative bacteria. Of the Vibrio species tested, more effective activity was detected against V. anguillarum. (C) 2010 Elsevier Ltd. All rights reserved.
关键词Haliotis Discus Hannai Ino Chicken-type Lysozyme Promoter Recombinant Protein Bacteriolytic Activity
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2010.10.008
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000286905800021
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/12027
专题海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位中国科学院海洋研究所
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Ding, Jianfeng,Li, Juan,Bao, Yongbo,et al. Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):163-172.
APA Ding, Jianfeng,Li, Juan,Bao, Yongbo,Li, Li,Wu, Fucun,&Zhang, Guofan.(2011).Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein.FISH & SHELLFISH IMMUNOLOGY,30(1),163-172.
MLA Ding, Jianfeng,et al."Molecular characterization of a mollusk chicken-type lysozyme gene from Haliotis discus hannai Ino, and the antimicrobial activity of its recombinant protein".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):163-172.
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