| Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system | |
| Zhang, Linlin1,2; Li, Li1; Zhang, Guofan1 | |
| 2011-05-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| 卷号 | 35期号:5页码:603-610 |
| 文章类型 | Article |
| 摘要 | Apoptosis system was reported to play important role in organism immunity, but it was a currently understudied respect in molluscan immunity studies. Base on the recent generation of ESTs in the pacific oyster. Crassostrea gigas, a survey of apoptosis-related molecules was conducted in the assembled uni-genes, we found that the basic genes and domains in apoptosis-associated proteins were conserved, the overall apoptotic machinery was complex in C. gigas and that the organism had an expanded number of putative baculovirus inhibitor of apoptosis repeat domains. Moreover, four typical apoptosis-related genes were cloned in C. gigas and compared with the sequences of these genes in Drosophila melanogaster and Homo sapiens. The expression level of these four apoptosis-related genes in the hemolymph increased dramatically in the presence of the bacteria, Vibrio anguillarum, indicating their role in bacterial defense. Our results suggest that the oyster apoptosis system is not simple and cannot be represented by model invertebrates. (C) 2011 Elsevier Ltd. All rights reserved.; Apoptosis system was reported to play important role in organism immunity, but it was a currently understudied respect in molluscan immunity studies. Base on the recent generation of ESTs in the pacific oyster. Crassostrea gigas, a survey of apoptosis-related molecules was conducted in the assembled uni-genes, we found that the basic genes and domains in apoptosis-associated proteins were conserved, the overall apoptotic machinery was complex in C. gigas and that the organism had an expanded number of putative baculovirus inhibitor of apoptosis repeat domains. Moreover, four typical apoptosis-related genes were cloned in C. gigas and compared with the sequences of these genes in Drosophila melanogaster and Homo sapiens. The expression level of these four apoptosis-related genes in the hemolymph increased dramatically in the presence of the bacteria, Vibrio anguillarum, indicating their role in bacterial defense. Our results suggest that the oyster apoptosis system is not simple and cannot be represented by model invertebrates. (C) 2011 Elsevier Ltd. All rights reserved. |
| 关键词 | Lophotrochozoa Apoptosis Evolution Crassostrea Gigas Bir Immune Response |
| 学科领域 | Immunology ; Zoology |
| DOI | 10.1016/j.dci.2011.01.005 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289333500011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12019 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Linlin,Li, Li,Zhang, Guofan. Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(5):603-610. |
| APA | Zhang, Linlin,Li, Li,&Zhang, Guofan.(2011).Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(5),603-610. |
| MLA | Zhang, Linlin,et al."Gene discovery, comparative analysis and expression profile reveal the complexity of the Crassostrea gigas apoptosis system".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.5(2011):603-610. |
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