| Cloning, genomic structure, and expression analysis of peroxiredoxin V from bay scallop Argopecten irradians | |
| Li, Juan1,2; Li, Li1; Zhang, Shoudu1,2; Zhang, Guofan1 | |
| 2011 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| 卷号 | 30期号:1页码:309-316 |
| 文章类型 | Article |
| 摘要 | Peroxiredoxins (Prxs) constitute a superfamily of antioxidative proteins that play important roles in protecting organisms against damage from reactive oxygen species (ROS). In this study, the peroxiredoxin V gene from Argopecten irradians (Ai-PrxV) was isolated and characterized. The full-length Ai-PrxV cDNA consists of 1689 bp with a 567 bp open reading frame (ORF) that encodes 188 amino acids. Three putative polyadenylation consensus signals (AATAAA) were found in the 953 bp long 3'-UTR. The genomic length of the Ai-PrxV gene is 12575 bp, and it contains six exons and five introns. The gene structure is closely related to those of chordates but differs from those of arthropods. The 5' flanking region, which contains several putative transcription factor binding sites, was also analyzed. Quantitative real-time PCR analysis showed that the highest expression of the Ai-PrxV transcripts occurred in gill tissue. When challenged with the bacteria Vibrio anguillarum, the level of Ai-PrxV transcripts in hemocytes of bay scallops was up-regulated and reached the highest point at 15 h post-challenge. These results indicate that Ai-PrxV is a constitutive and inducible protein that plays an important role in the immune response against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Peroxiredoxins (Prxs) constitute a superfamily of antioxidative proteins that play important roles in protecting organisms against damage from reactive oxygen species (ROS). In this study, the peroxiredoxin V gene from Argopecten irradians (Ai-PrxV) was isolated and characterized. The full-length Ai-PrxV cDNA consists of 1689 bp with a 567 bp open reading frame (ORF) that encodes 188 amino acids. Three putative polyadenylation consensus signals (AATAAA) were found in the 953 bp long 3'-UTR. The genomic length of the Ai-PrxV gene is 12575 bp, and it contains six exons and five introns. The gene structure is closely related to those of chordates but differs from those of arthropods. The 5' flanking region, which contains several putative transcription factor binding sites, was also analyzed. Quantitative real-time PCR analysis showed that the highest expression of the Ai-PrxV transcripts occurred in gill tissue. When challenged with the bacteria Vibrio anguillarum, the level of Ai-PrxV transcripts in hemocytes of bay scallops was up-regulated and reached the highest point at 15 h post-challenge. These results indicate that Ai-PrxV is a constitutive and inducible protein that plays an important role in the immune response against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. |
| 关键词 | Argopecten Irradians Peroxiredoxin v (Prxv) Cloning Gene Structure Immune Response |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2010.11.011 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286905800039 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12001 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Juan,Li, Li,Zhang, Shoudu,et al. Cloning, genomic structure, and expression analysis of peroxiredoxin V from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):309-316. |
| APA | Li, Juan,Li, Li,Zhang, Shoudu,&Zhang, Guofan.(2011).Cloning, genomic structure, and expression analysis of peroxiredoxin V from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,30(1),309-316. |
| MLA | Li, Juan,et al."Cloning, genomic structure, and expression analysis of peroxiredoxin V from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):309-316. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Cloning genomic stru（1616KB） | 限制开放 | -- | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论