The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence

doi:10.1371/journal.pone.0014663

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	The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence
	Lue, Fang 1,3; Xue, Wei 2,3; Tian, Chao 1; Wang, Guangce 1; Niu, Jiangfeng 1; Pan, Guanghua 4; Hu, Songnian 3
	2011-02-14
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	6 期号:2 页码:e14663
文章类型	Article
摘要	Background: Bryopsis hypnoides Lamouroux is a siphonous green alga, and its extruded protoplasm can aggregate spontaneously in seawater and develop into mature individuals. The chloroplast of B. hypnoides is the biggest organelle in the cell and shows strong autonomy. To better understand this organelle, we sequenced and analyzed the chloroplast genome of this green alga.; Background: Bryopsis hypnoides Lamouroux is a siphonous green alga, and its extruded protoplasm can aggregate spontaneously in seawater and develop into mature individuals. The chloroplast of B. hypnoides is the biggest organelle in the cell and shows strong autonomy. To better understand this organelle, we sequenced and analyzed the chloroplast genome of this green alga. Principal Findings: A total of 111 functional genes, including 69 potential protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes were identified. The genome size (153,429 bp), arrangement, and inverted-repeat (IR)-lacking structure of the B. hypnoides chloroplast DNA (cpDNA) closely resembles that of Chlorella vulgaris. Furthermore, our cytogenomic investigations using pulsed-field gel electrophoresis (PFGE) and southern blotting methods showed that the B. hypnoides cpDNA had multimeric forms, including monomer, dimer, trimer, tetramer, and even higher multimers, which is similar to the higher order organization observed previously for higher plant cpDNA. The relative amounts of the four multimeric cpDNA forms were estimated to be about 1, 1/2, 1/4, and 1/8 based on molecular hybridization analysis. Phylogenetic analyses based on a concatenated alignment of chloroplast protein sequences suggested that B. hypnoides is sister to all Chlorophyceae and this placement received moderate support. Conclusion: All of the results suggest that the autonomy of the chloroplasts of B. hypnoides has little to do with the size and gene content of the cpDNA, and the IR-lacking structure of the chloroplasts indirectly demonstrated that the multimeric molecules might result from the random cleavage and fusion of replication intermediates instead of recombinational events.
学科领域	Life Sciences & Biomedicine - Other Topics
DOI	10.1371/journal.pone.0014663
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000287367600003
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11957
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Beijing Genom Inst, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lue, Fang,Xue, Wei,Tian, Chao,et al. The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence[J]. PLOS ONE,2011,6(2):e14663.
APA	Lue, Fang.,Xue, Wei.,Tian, Chao.,Wang, Guangce.,Niu, Jiangfeng.,...&Hu, Songnian.(2011).The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence.PLOS ONE,6(2),e14663.
MLA	Lue, Fang,et al."The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence".PLOS ONE 6.2(2011):e14663.

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