Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Suppressor of cytokine signaling 3 inhibits head kidney macrophage activation and cytokine expression in Scophthalmus maximus | |
| Zhang, Min; Xiao, Zhi-zhong; Sun, Li | |
| 2011-02-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| 卷号 | 35期号:2页码:174-181 |
| 文章类型 | Article |
| 摘要 | Proteins of the suppressor of cytokine signaling (SOCS) family function as inducible feedback inhibitors of cytokine signaling via the JAK/STAT pathway. Although several SOCS isoforms have been identified in teleosts, their immunological functions remain largely unknown. In this study, we identified in turbot Scophthalmus maximus a SOCS homologue (named SmSOCS3) of the mammalian SOCS3 type. The deduced amino acid sequence of SmSOCS3 contains 205 residues and shares extensive overall identities (60-82%) with those of known fish SOCS3. In silica analyses revealed that, like typical SOCS3. SmSOCS3 possesses a kinase inhibitor region (KIR). a Src homology 2 (SH2) domain, and a SOCS box domain. Under physiological conditions SmSOCS3 expression was detected, in increasing order, in blood, brain, heart, kidney, liver, spleen, muscle, and gill. Experimental infection of turbot with a bacterial pathogen induced significant SmSOCS3 expression in kidney, spleen, liver, and gill in time-dependent manners. Examination of SmSOCS3 expression in head kidney (HK) macrophages showed that SmSOCS3 transcription was significantly upregulated in the presence of purified recombinant TNF-alpha. On the other hand, SmSOCS3 overexpression in HK macrophages inhibited the transcription of TNF-alpha as well as IL-1 beta and CC-chemokine. In addition, SmSOCS3 overexpression significantly reduced macrophage respiratory burst activity, nitric oxide production, and bactericidal activity. Taken together, these results suggest that SmSOCS3 is a cytokine-inducible suppressor of pro-inflammatory cytokine signaling in HK macrophages and that regulated expression of SmSOCS3 is required for optimal innate immune response against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Proteins of the suppressor of cytokine signaling (SOCS) family function as inducible feedback inhibitors of cytokine signaling via the JAK/STAT pathway. Although several SOCS isoforms have been identified in teleosts, their immunological functions remain largely unknown. In this study, we identified in turbot Scophthalmus maximus a SOCS homologue (named SmSOCS3) of the mammalian SOCS3 type. The deduced amino acid sequence of SmSOCS3 contains 205 residues and shares extensive overall identities (60-82%) with those of known fish SOCS3. In silica analyses revealed that, like typical SOCS3. SmSOCS3 possesses a kinase inhibitor region (KIR). a Src homology 2 (SH2) domain, and a SOCS box domain. Under physiological conditions SmSOCS3 expression was detected, in increasing order, in blood, brain, heart, kidney, liver, spleen, muscle, and gill. Experimental infection of turbot with a bacterial pathogen induced significant SmSOCS3 expression in kidney, spleen, liver, and gill in time-dependent manners. Examination of SmSOCS3 expression in head kidney (HK) macrophages showed that SmSOCS3 transcription was significantly upregulated in the presence of purified recombinant TNF-alpha. On the other hand, SmSOCS3 overexpression in HK macrophages inhibited the transcription of TNF-alpha as well as IL-1 beta and CC-chemokine. In addition, SmSOCS3 overexpression significantly reduced macrophage respiratory burst activity, nitric oxide production, and bactericidal activity. Taken together, these results suggest that SmSOCS3 is a cytokine-inducible suppressor of pro-inflammatory cytokine signaling in HK macrophages and that regulated expression of SmSOCS3 is required for optimal innate immune response against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. |
| 关键词 | Suppressor Of Cytokine Signaling Scophthalmus Maximus Macrophage Innate Immune Response |
| 学科领域 | Immunology ; Zoology |
| DOI | 10.1016/j.dci.2010.09.006 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285127400004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11951 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Xiao, Zhi-zhong,Sun, Li. Suppressor of cytokine signaling 3 inhibits head kidney macrophage activation and cytokine expression in Scophthalmus maximus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(2):174-181. |
| APA | Zhang, Min,Xiao, Zhi-zhong,&Sun, Li.(2011).Suppressor of cytokine signaling 3 inhibits head kidney macrophage activation and cytokine expression in Scophthalmus maximus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(2),174-181. |
| MLA | Zhang, Min,et al."Suppressor of cytokine signaling 3 inhibits head kidney macrophage activation and cytokine expression in Scophthalmus maximus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.2(2011):174-181. |
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