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| Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803 | |
| Liang, Chengwei1,2; Zhang, Xiaowen2,3; Chi, Xiaoyuan2; Guan, Xiangyu4; Li, Youxun2; Qin, Song2; Shao, Hong Bo5 | |
| 2011-05-26 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 6期号:5页码:e18718 |
| 文章类型 | Article |
| 摘要 | Background: Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803.; Background: Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803. Methodology/Principal Findings: In this paper, we examined differential expression of the other four serine/threonine kinases, SpkC, SpkD, SpkF and SpkG, at seven different stress conditions. The transcriptional level was up-regulated of spkG and down-regulated of spkC under high salt stress condition. Two spk deletion mutants, Delta spkC and Delta spkG, were constructed and their growth characteristic were examined compared to the wild strain. The wild strain and Delta spkC mutant were not affected under high salt stress conditions. In contrast, growth of spkG mutant was completely impaired. To further confirm the function of spkG, we also examined the effect of mutation of spkG on the expression of salt stress-inducible genes. We compared genome-wide patterns of transcription between wild-type Synechocystis sp. PCC6803 and cells with a mutation in the SpkG with DNA microarray analysis. Conclusion: In this study, we first study the spkG gene as sensor of high salt signal. We consider that SpkG play essential roles in Synechocystis sp. for sensing the high salt signal directly, rather than mediating signals among other kinases. Our microarray experiment may help select relatively significant genes for further research on mechanisms of signal transduction of Synechocystis sp. PCC6803 under high salt stress. |
| 学科领域 | Life Sciences & Biomedicine - Other Topics |
| DOI | 10.1371/journal.pone.0018718 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291052200008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11941 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Qingdao Univ Sci & Technol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China 4.Ocean Univ China, Qingdao, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liang, Chengwei,Zhang, Xiaowen,Chi, Xiaoyuan,et al. Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803[J]. PLOS ONE,2011,6(5):e18718. |
| APA | Liang, Chengwei.,Zhang, Xiaowen.,Chi, Xiaoyuan.,Guan, Xiangyu.,Li, Youxun.,...&Shao, Hong Bo.(2011).Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803.PLOS ONE,6(5),e18718. |
| MLA | Liang, Chengwei,et al."Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803".PLOS ONE 6.5(2011):e18718. |
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