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Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles
Chen, Yingjie1,2; Jiang, Peng1; Liu, Shaofang1,2; Zhao, Hui1,2; Cui, Yulin1,2; Qin, Song1
2011-04-15
发表期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
卷号879期号:13-14页码:993-997
文章类型Article
摘要Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6xHis-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6xHis-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25 degrees C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6xHis-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6xHis-tagged phycobiliprotein. (C) 2011 Elsevier B.V. All rights reserved.; Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6xHis-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6xHis-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25 degrees C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6xHis-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6xHis-tagged phycobiliprotein. (C) 2011 Elsevier B.V. All rights reserved.
关键词6xhis Tag Phycobiliprotein Silica-coated Magnetic Nanoparticles (Simnps) Zn(Ii) Purification Adsorption Behavior
学科领域Biochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.jchromb.2011.03.014
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收录类别SCI
语种英语
WOS记录号WOS:000290005400025
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/11925
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Chen, Yingjie,Jiang, Peng,Liu, Shaofang,et al. Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(13-14):993-997.
APA Chen, Yingjie,Jiang, Peng,Liu, Shaofang,Zhao, Hui,Cui, Yulin,&Qin, Song.(2011).Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(13-14),993-997.
MLA Chen, Yingjie,et al."Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.13-14(2011):993-997.
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