Potential Role of Cathepsin B in the Embryonic and Larval Development of Clam Meretrix meretrix

doi:10.1002/jez.b.21402

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	Potential Role of Cathepsin B in the Embryonic and Larval Development of Clam Meretrix meretrix
	Wang, Xiaomei 1,2; Liu, Baozhong1 ; Tang, Baojun 3; Xiang, Jianhai1
	2011-06-15
发表期刊	JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
ISSN	1552-5007
卷号	316B 期号:4 页码:306-312
文章类型	Article
摘要	This study was designed to investigate the possible role of Meretrix meretrix cathepsin B (MmeCB) in embryonic and larval development. MmeCB mRNA expression profile was revealed by semi-quantitative RT-PCR. The level of MmeCB mRNA expression was low in trochophore stage but high in pedveliger stage. MmeCB protein expression was detected in the digestive gland, velum, and epidermis along the edges of the shell in D-larvae and pedveligers by immunocytochemistry. In post larvae, MmeCB protein expression was noticed abundant in the digestive gland, whereas a modest expression was identified in the gill filament. The average shell length of larvae hatched from embryos treated with 0.01, 1, and 10 mu mol/L Ca074Me (a cathepsin B inhibitor) was significantly shorter than that of control groups. The metamorphosis rates of larvae treated with 0.01 and 1 mu mol/L Ca074Me were significantly lower than that of control groups in 4-day larvae, but not in 5-day larvae. Taken together, these results indicated that MmeCB may have stimulatory effects on embryonic development, metamorphosis, and larval growth during M. meretrix larval development. J. Exp. Zool. (Mol. Dev. Evol.) 316:306-312, 2011. (C) 2011 Wiley-Liss, Inc.; This study was designed to investigate the possible role of Meretrix meretrix cathepsin B (MmeCB) in embryonic and larval development. MmeCB mRNA expression profile was revealed by semi-quantitative RT-PCR. The level of MmeCB mRNA expression was low in trochophore stage but high in pedveliger stage. MmeCB protein expression was detected in the digestive gland, velum, and epidermis along the edges of the shell in D-larvae and pedveligers by immunocytochemistry. In post larvae, MmeCB protein expression was noticed abundant in the digestive gland, whereas a modest expression was identified in the gill filament. The average shell length of larvae hatched from embryos treated with 0.01, 1, and 10 mu mol/L Ca074Me (a cathepsin B inhibitor) was significantly shorter than that of control groups. The metamorphosis rates of larvae treated with 0.01 and 1 mu mol/L Ca074Me were significantly lower than that of control groups in 4-day larvae, but not in 5-day larvae. Taken together, these results indicated that MmeCB may have stimulatory effects on embryonic development, metamorphosis, and larval growth during M. meretrix larval development. J. Exp. Zool. (Mol. Dev. Evol.) 316:306-312, 2011. (C) 2011 Wiley-Liss, Inc.
学科领域	Evolutionary Biology ; Developmental Biology ; Zoology
DOI	10.1002/jez.b.21402
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000290546300007
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11921
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, Beijing, Peoples R China 3.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Xiaomei,Liu, Baozhong,Tang, Baojun,et al. Potential Role of Cathepsin B in the Embryonic and Larval Development of Clam Meretrix meretrix[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,2011,316B(4):306-312.
APA	Wang, Xiaomei,Liu, Baozhong,Tang, Baojun,&Xiang, Jianhai.(2011).Potential Role of Cathepsin B in the Embryonic and Larval Development of Clam Meretrix meretrix.JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,316B(4),306-312.
MLA	Wang, Xiaomei,et al."Potential Role of Cathepsin B in the Embryonic and Larval Development of Clam Meretrix meretrix".JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION 316B.4(2011):306-312.

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