Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration

doi:10.1007/s00343-011-0028-4

IOCAS-IR > 实验海洋生物学重点实验室

	Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration
	Lue Fang 1,2; Wang Guangce 1,3; Jin Haochen 3
	2011-03-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	29 期号:2 页码:334-342
文章类型	Article
摘要	Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this study, the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B. hypnoides were measured using a Dual-PAM (pulse amplitude modulation)-100 fluorometer. The results show that the photosynthetic rates of B. hypnoides thalli were maintained for an initial period, beyond which continued desiccation resulted in reduced rates of PSI and PSII. However, the photosynthetic performances of the isolated protoplasts dehydrated in air (CO(2) concentration 600-700 mg/L) showed a slight increase of Y(II) at 20% water loss, but the rates decreased thereafter with declining water content. When protoplasts were dehydrated in CO(2) deficient conditions (CO(2) concentration 40 80 mg/L), the values of Y(II) declined steadily with increased dehydration without an initial rise. These results indicated that the thalli and isolated protoplasts of this alga can utilize CO(2) in ambient air effectively, and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration. Thus the protoplasts may be an excellent system for the study of stress tolerance.; Bryopsis hypnoides Lamouroux is a unique intertidal siphonous green alga whose extruded protoplasm can aggregate spontaneously in seawater to form numerous new cells that can develop into mature algal thalli. In this study, the photosynthetic responses during dehydration of both the thalli and protoplasts isolated from B. hypnoides were measured using a Dual-PAM (pulse amplitude modulation)-100 fluorometer. The results show that the photosynthetic rates of B. hypnoides thalli were maintained for an initial period, beyond which continued desiccation resulted in reduced rates of PSI and PSII. However, the photosynthetic performances of the isolated protoplasts dehydrated in air (CO(2) concentration 600-700 mg/L) showed a slight increase of Y(II) at 20% water loss, but the rates decreased thereafter with declining water content. When protoplasts were dehydrated in CO(2) deficient conditions (CO(2) concentration 40 80 mg/L), the values of Y(II) declined steadily with increased dehydration without an initial rise. These results indicated that the thalli and isolated protoplasts of this alga can utilize CO(2) in ambient air effectively, and the photosynthetic performances of the isolated protoplasts were significantly different from that of the thalli during dehydration. Thus the protoplasts may be an excellent system for the study of stress tolerance.
关键词	Bryopsis Hypnoides Chlorophyll Fluorescence Dehydration Protoplasts Pulse Amplified Modulation Fluorescence System
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-011-0028-4
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000287924000013
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11915
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Lue Fang,Wang Guangce,Jin Haochen. Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(2):334-342.
APA	Lue Fang,Wang Guangce,&Jin Haochen.(2011).Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(2),334-342.
MLA	Lue Fang,et al."Photosynthetic responses of thalli and isolated protoplasts of Bryopsis hypnoides (Bryopsidales, Chlorophyta) during dehydration".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.2(2011):334-342.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Photosynthetic respo（162KB）			限制开放	--	浏览