Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property

doi:10.1016/j.fsi.2011.03.023

IOCAS-IR > 实验海洋生物学重点实验室

	Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property
	Sun, Jin-sheng 2; Zhao, Lu 1,2; Sun, Li 1
	2011-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	30 期号:6 页码:1362-1367
文章类型	Article
摘要	Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1 beta and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response. (C) 2011 Elsevier Ltd. All rights reserved.; Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1 beta and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Interleukin-8 Cynoglossus Semilaevis Inflammation Immunoregulatory
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2011.03.023
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000291838400018
引用统计	被引频次：60[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11881
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jin-sheng,Zhao, Lu,Sun, Li. Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(6):1362-1367.
APA	Sun, Jin-sheng,Zhao, Lu,&Sun, Li.(2011).Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property.FISH & SHELLFISH IMMUNOLOGY,30(6),1362-1367.
MLA	Sun, Jin-sheng,et al."Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property".FISH & SHELLFISH IMMUNOLOGY 30.6(2011):1362-1367.

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