Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification and characterization of microRNAs from Phaeodactylum tricornutum by high-throughput sequencing and bioinformatics analysis | |
| Huang, Aiyou1,2; He, Linwen1,2; Wang, Guangce1 | |
| 2011-06-30 | |
| 发表期刊 | BMC GENOMICS
 |
| ISSN | 1471-2164 |
| 卷号 | 12页码:11 |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 摘要 | Background: Diatoms, which are important planktons widespread in various aquatic environments, are believed to play a vital role in primary production as well as silica cycling. The genomes of the pennate diatom Phaeodactylum tricornutum and the centric diatom Thalassiosira pseudonana have been sequenced, revealing some characteristics of the diatoms' mosaic genome as well as some features of their fatty acid metabolism and urea cycle, and indicating their unusual properties. To identify microRNAs (miRNAs) from P. tricornutum and to study their probable roles in nitrogen and silicon metabolism, we constructed and sequenced small RNA (sRNA) libraries from P. tricornutum under normal (PT1), nitrogen-limited (PT2) and silicon-limited (PT3) conditions. Results: A total of 13 miRNAs were identified. They were probable P. tricornutum-specific novel miRNAs. These miRNAs were sequenced from P. tricornutum under normal, nitrogen-limited and/or silicon-limited conditions, and their potential targets were involved in various processes, such as signal transduction, protein amino acid phosphorylation, fatty acid biosynthetic process, regulation of transcription and so on. Conclusions: Our results indicated that P. tricornutum contained novel miRNAs that have no identifiable homologs in other organisms and that they might play important regulator roles in P. tricornutum metabolism. |
| DOI | 10.1186/1471-2164-12-337 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[30830015] ; National Natural Science Foundation of China[30970302] ; National Natural Science Foundation of China[40806063] ; National Natural Science Foundation of China[B49082401] ; Innovative Foundation of Chinese Academy of Sciences[KGCX2-YW-374-3] ; National Natural Science Foundation of China[30830015] ; National Natural Science Foundation of China[30970302] ; National Natural Science Foundation of China[40806063] ; National Natural Science Foundation of China[B49082401] ; Innovative Foundation of Chinese Academy of Sciences[KGCX2-YW-374-3] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS记录号 | WOS:000292985900001 |
| 出版者 | BIOMED CENTRAL LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11871 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Sch Earth Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Aiyou,He, Linwen,Wang, Guangce. Identification and characterization of microRNAs from Phaeodactylum tricornutum by high-throughput sequencing and bioinformatics analysis[J]. BMC GENOMICS,2011,12:11. |
| APA | Huang, Aiyou,He, Linwen,&Wang, Guangce.(2011).Identification and characterization of microRNAs from Phaeodactylum tricornutum by high-throughput sequencing and bioinformatics analysis.BMC GENOMICS,12,11. |
| MLA | Huang, Aiyou,et al."Identification and characterization of microRNAs from Phaeodactylum tricornutum by high-throughput sequencing and bioinformatics analysis".BMC GENOMICS 12(2011):11. |
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