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| CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells | |
| Cheng, Linyou1,2; Liu, Ming1; Wang, Cuicui1,2; Liu, Haizhou1,3; Zhang, Yuyan1,3; Lin, Xiukun1 | |
| 2011 | |
| 发表期刊 | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
 |
| ISSN | 1741-427X |
| 页码 | 1-9 |
| 文章类型 | Article |
| 摘要 | In the present studies, a novel compound with potent anti-tumor activity from Ciona intestinalis L. was purified by acetone fractionation, ultrafiltration, gel chromatography and High Performance Liquid Chromatography. The molecular weight of the highly purified compound, designated CI431, was 431Da as determined by HPLC-MS analysis. CI431 exhibited significant cytotoxicity to several cancer cell types. However, only a slight inhibitory effect was found when treating the benign human liver cell line BEL-7702 with the compound. To explore its mechanism against hepatocellular carcinoma, BEL-7402 cells were treated with CI431 in vitro. We found that CI431 induced apoptotic death in BEL-7402 cells in a dose-and time-dependent manner. Cell cycle analysis demonstrated that CI431 caused cell cycle arrest at the G2/ M phase, and a sub-G1 peak appeared after 24 h. The mitochondrial-mediated pathway was implicated in this CI431-induced apoptosis as evidenced by the disruption of mitochondrial membrane potential. The results suggest that the CI431 induces apoptosis in BEL-7402 human hepatoma cells by intrinsic mitochondrial pathway.; In the present studies, a novel compound with potent anti-tumor activity from Ciona intestinalis L. was purified by acetone fractionation, ultrafiltration, gel chromatography and High Performance Liquid Chromatography. The molecular weight of the highly purified compound, designated CI431, was 431Da as determined by HPLC-MS analysis. CI431 exhibited significant cytotoxicity to several cancer cell types. However, only a slight inhibitory effect was found when treating the benign human liver cell line BEL-7702 with the compound. To explore its mechanism against hepatocellular carcinoma, BEL-7402 cells were treated with CI431 in vitro. We found that CI431 induced apoptotic death in BEL-7402 cells in a dose-and time-dependent manner. Cell cycle analysis demonstrated that CI431 caused cell cycle arrest at the G2/ M phase, and a sub-G1 peak appeared after 24 h. The mitochondrial-mediated pathway was implicated in this CI431-induced apoptosis as evidenced by the disruption of mitochondrial membrane potential. The results suggest that the CI431 induces apoptosis in BEL-7402 human hepatoma cells by intrinsic mitochondrial pathway. |
| 学科领域 | Integrative & Complementary Medicine |
| DOI | 10.1155/2011/292873 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293408500001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11799 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Acad Sinica, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, Linyou,Liu, Ming,Wang, Cuicui,et al. CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2011:1-9. |
| APA | Cheng, Linyou,Liu, Ming,Wang, Cuicui,Liu, Haizhou,Zhang, Yuyan,&Lin, Xiukun.(2011).CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,1-9. |
| MLA | Cheng, Linyou,et al."CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2011):1-9. |
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