Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors

doi:10.1007/s11626-011-9406-7

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	Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors
	Zhang, Yuqing 1,2; Tan, Xungang1 ; Sun, Wei 1,2; Xu, Peng1,2 ; Zhang, Pei-Jun 1; Xu, Yongli 1
	2011-06-01
发表期刊	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
ISSN	1071-2690
卷号	47 期号:5-6 页码:399-405
文章类型	Article
摘要	As one member of winged helix domain transcription factors, FoxD3 plays an important role in the regulation of neural crest development and maintenance of mammalian stem cell lineages. A recent study showed that zebrafish FoxD3 is a downstream gene of Pax3 and can mediate the expression of Myf5. To further understand the function of FoxD3 in fish muscle development, we isolated the FoxD3 gene from flounder, and analyzed its expression pattern and function in regulating myogenic regulatory factors, MyoD and Myf5. In situ hybridization showed that flounder FoxD3 was firstly detected in the premigratory neural crest cells at 90% epiboly stage. The FoxD3 was expressed not only in neural crest cells but also in somite cells that will form muscle in the future. When flounder FoxD3 was over-expressed in zebrafish by microinjection, the expressions of zebrafish Myf5 and MyoD were both affected. It is possible that FoxD3 is involved in myogenesis by regulating the expression of Myf5 and MyoD. Also, over-expression of flounder FoxD3 in zebrafish inhibits the expression of zebrafish endogenic FoxD3.; As one member of winged helix domain transcription factors, FoxD3 plays an important role in the regulation of neural crest development and maintenance of mammalian stem cell lineages. A recent study showed that zebrafish FoxD3 is a downstream gene of Pax3 and can mediate the expression of Myf5. To further understand the function of FoxD3 in fish muscle development, we isolated the FoxD3 gene from flounder, and analyzed its expression pattern and function in regulating myogenic regulatory factors, MyoD and Myf5. In situ hybridization showed that flounder FoxD3 was firstly detected in the premigratory neural crest cells at 90% epiboly stage. The FoxD3 was expressed not only in neural crest cells but also in somite cells that will form muscle in the future. When flounder FoxD3 was over-expressed in zebrafish by microinjection, the expressions of zebrafish Myf5 and MyoD were both affected. It is possible that FoxD3 is involved in myogenesis by regulating the expression of Myf5 and MyoD. Also, over-expression of flounder FoxD3 in zebrafish inhibits the expression of zebrafish endogenic FoxD3.
关键词	Flounder Foxd3 Muscle Over-expression In Situ Hybridization Myogenic Regulatory Factors
学科领域	Cell Biology ; Developmental Biology
DOI	10.1007/s11626-011-9406-7
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000291256200010
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11795
专题	实验海洋生物学重点实验室海洋环流与波动重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Yuqing,Tan, Xungang,Sun, Wei,et al. Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(5-6):399-405.
APA	Zhang, Yuqing,Tan, Xungang,Sun, Wei,Xu, Peng,Zhang, Pei-Jun,&Xu, Yongli.(2011).Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(5-6),399-405.
MLA	Zhang, Yuqing,et al."Characterization of flounder (Paralichthys olivaceus) FoxD3 and its function in regulating myogenic regulatory factors".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.5-6(2011):399-405.

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