Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix | |
Lu, Xia1,2; Wang, Hongxia1; Dai, Ping1,2; Liu, Baozhong1 | |
2011-10-01 | |
发表期刊 | CONSERVATION GENETICS RESOURCES |
ISSN | 1877-7252 |
卷号 | 3期号:4页码:655-658 |
文章类型 | Article |
摘要 | We described and characterized 17 expressed sequence tag (EST)-derived simple sequence repeats (SSR) and 16 genomic-derived SSRs for the clam Meretrix meretrix. The EST-SSRs comprised 17.6% dinucleotide repeats, 35.3% trinucleotide repeats, and 47.1% tetranucleotide repeats; whereas the majority of the G-SSRs were dinuleotide repeats (75.0%). The number of alleles for the 33 SSR markers varied from 6 to 15, with a mean of 10.3 alleles per locus. Observed and expected heterozygosity (Ho and He) varied from 0.613 to 0.824, and 0.687 to 0.850, respectively. Polymorphic information content (PIC) ranged from 0.645 to 0.842. The mean values of number of alleles, Ho, He, and PIC of G-SSRs were higher than those of the EST-SSRs. No significant deviations from Hardy-Weinberg equilibrium or linkage disequilibrium were observed in the 33 loci. These loci are the first to be characterized in M. meretrix and should be useful in the investigation of genetic evaluation for conservation.; We described and characterized 17 expressed sequence tag (EST)-derived simple sequence repeats (SSR) and 16 genomic-derived SSRs for the clam Meretrix meretrix. The EST-SSRs comprised 17.6% dinucleotide repeats, 35.3% trinucleotide repeats, and 47.1% tetranucleotide repeats; whereas the majority of the G-SSRs were dinuleotide repeats (75.0%). The number of alleles for the 33 SSR markers varied from 6 to 15, with a mean of 10.3 alleles per locus. Observed and expected heterozygosity (Ho and He) varied from 0.613 to 0.824, and 0.687 to 0.850, respectively. Polymorphic information content (PIC) ranged from 0.645 to 0.842. The mean values of number of alleles, Ho, He, and PIC of G-SSRs were higher than those of the EST-SSRs. No significant deviations from Hardy-Weinberg equilibrium or linkage disequilibrium were observed in the 33 loci. These loci are the first to be characterized in M. meretrix and should be useful in the investigation of genetic evaluation for conservation. |
关键词 | Est-ssr Genomic-ssr Microsatellite Clam Meretrix Meretrix |
学科领域 | Biodiversity & Conservation ; Genetics & Heredity |
DOI | 10.1007/s12686-011-9426-3 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000294682100014 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/11793 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
第一作者单位 | 实验海洋生物学重点实验室 |
推荐引用方式 GB/T 7714 | Lu, Xia,Wang, Hongxia,Dai, Ping,et al. Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix[J]. CONSERVATION GENETICS RESOURCES,2011,3(4):655-658. |
APA | Lu, Xia,Wang, Hongxia,Dai, Ping,&Liu, Baozhong.(2011).Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix.CONSERVATION GENETICS RESOURCES,3(4),655-658. |
MLA | Lu, Xia,et al."Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix".CONSERVATION GENETICS RESOURCES 3.4(2011):655-658. |
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