Biosynthesis and Immobilization of Biofunctional Allophycocyanin

doi:10.1155/2011/751452

IOCAS-IR > 实验海洋生物学重点实验室

	Biosynthesis and Immobilization of Biofunctional Allophycocyanin
	Chen, Yingjie 1,2; Liu, Shaofang 1,2; Cui, Yulin 1,2; Jiang, Peng 1; Chen, Huaxin 1; Li, Fuchao 1; Qin, Song 1
	2011
发表期刊	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
ISSN	1741-427X
页码	751452
文章类型	Article
摘要	The holo-allophycocyanin-a subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles. The holo-allophycocyanin-a subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles. Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.; The holo-allophycocyanin-a subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles. The holo-allophycocyanin-a subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles. Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.
学科领域	Integrative & Complementary Medicine
DOI	10.1155/2011/751452
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000295672500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11787
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen, Yingjie,Liu, Shaofang,Cui, Yulin,et al. Biosynthesis and Immobilization of Biofunctional Allophycocyanin[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2011:751452.
APA	Chen, Yingjie.,Liu, Shaofang.,Cui, Yulin.,Jiang, Peng.,Chen, Huaxin.,...&Qin, Song.(2011).Biosynthesis and Immobilization of Biofunctional Allophycocyanin.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,751452.
MLA	Chen, Yingjie,et al."Biosynthesis and Immobilization of Biofunctional Allophycocyanin".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2011):751452.

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