An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity

doi:10.1016/j.fsi.2010.11.022

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	An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity
	Yue, Xin 1,2; Liu, Baozhong1 ; Xue, Qinggang 3
	2011-02-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	30 期号:2 页码:550-558
文章类型	Article
摘要	Lysozymes function in animal immunity. Three types of lysozyme\ have been identified in animal kingdom and most lysozymes identified from bivalve molluscs belong to the invertebrate (i) type. In this research, we cloned and sequenced a new i-type lysozyme, named MmeLys, from the Asiatic hard clam Meretrix meretrix. MmeLys cDNA was constituted of 552 bp, with a 441 bp open reading frame encoding a 146 amino acid polypeptide. The encoded polypeptide was predicted to have a 15 amino acid signal peptide, and a 131 amino acid mature protein with a theoretical mass of 14601.44 Da and an isoelectric point (pl) of 7.14. MmeLys amino acid sequence bore 64% identity with the Manila clam (Venerupis philippinarum) i-type lysozyme and was grouped with other veneroid i-type lysozymes in a bivalve lysozyme phylogenetic tree predicted using Neighbor-Jointing method. Recombinantly expressed MmeLys showed lysozyme activity and strong antibacterial activity against Gram positive and Gram negative bacteria. MmeLys mRNA and protein were detected to be mainly produced in hepatopancreas and gill by the methods of semi-quantitative RT-PCR and western blotting. In addition, MmeLys gene expression increased following Vibrio parahaemolyticus challenge. Results of this research indicated that MmeLys represents a new i-type lysozyme that likely functions in M. meretrix immunity. (C) 2010 Elsevier Ltd. All rights reserved.; Lysozymes function in animal immunity. Three types of lysozyme\ have been identified in animal kingdom and most lysozymes identified from bivalve molluscs belong to the invertebrate (i) type. In this research, we cloned and sequenced a new i-type lysozyme, named MmeLys, from the Asiatic hard clam Meretrix meretrix. MmeLys cDNA was constituted of 552 bp, with a 441 bp open reading frame encoding a 146 amino acid polypeptide. The encoded polypeptide was predicted to have a 15 amino acid signal peptide, and a 131 amino acid mature protein with a theoretical mass of 14601.44 Da and an isoelectric point (pl) of 7.14. MmeLys amino acid sequence bore 64% identity with the Manila clam (Venerupis philippinarum) i-type lysozyme and was grouped with other veneroid i-type lysozymes in a bivalve lysozyme phylogenetic tree predicted using Neighbor-Jointing method. Recombinantly expressed MmeLys showed lysozyme activity and strong antibacterial activity against Gram positive and Gram negative bacteria. MmeLys mRNA and protein were detected to be mainly produced in hepatopancreas and gill by the methods of semi-quantitative RT-PCR and western blotting. In addition, MmeLys gene expression increased following Vibrio parahaemolyticus challenge. Results of this research indicated that MmeLys represents a new i-type lysozyme that likely functions in M. meretrix immunity. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Invertebrate Lysozyme Meretrix Meretrix Recombinant Expression Antimicrobial Activity Immunity
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2010.11.022
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000288305300014
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11781
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Louisiana State Univ, Dept Vet Sci, Ctr Agr, Baton Rouge, LA 70803 USA
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yue, Xin,Liu, Baozhong,Xue, Qinggang. An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(2):550-558.
APA	Yue, Xin,Liu, Baozhong,&Xue, Qinggang.(2011).An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity.FISH & SHELLFISH IMMUNOLOGY,30(2),550-558.
MLA	Yue, Xin,et al."An i-type lysozyme from the Asiatic hard clam Meretrix meretrix potentially functioning in host immunity".FISH & SHELLFISH IMMUNOLOGY 30.2(2011):550-558.

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